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Lactate dehydrogenase, Catalase, and Superoxide dismutase in Tumor Tissue of Breast Cancer Patients in Respect to Mammographic Findings

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Abstract

Lactate dehydrogenase (LDH), marker of anaerobic metabolism, is associated with highly invasive and metastatic breast cancer. Novel studies show that increased anaerobic metabolism (LDH), as well as activity of antioxidative enzymes (superoxide dismutase (SOD) and catalase (CAT)), is correlated with higher mammographic density, as known predictor of breast cancer risk. In this study, we measured LDH, MDH, and SOD activity in tumor and adjacent tissues of breast cancer patients by spectrophotometric assay. Mammograms were evaluated according to the American College of Radiology Breast Imaging Reporting and Data system. Mammographically dense breast tissue is associated with higher activity of LDH in tumor tissue of breast cancer patients. Moreover, patients with masses have significantly higher activity of LDH compared to patients with focal asymmetries or architectural distortion. Patients with spiculated mass margin had higher activity of LDH compared to patients with focal asymmetries or architectural distortion. Activity of LDH in patients significantly increases, while activity of CAT significantly decreases with the increase of BIRADS category. These results suggest that the association of activity of LDH and CAT in tumor tissue with mammographic characteristics could help in defining aggressive breast cancers.

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Abbreviations

LDH:

Lactate dehydrogenase

SOD:

Superoxide dismutase

CAT:

Catalase

HER2:

Human epidermal growth factor receptor 2

DAB-3:

3Diaminobenzidine

ER:

Estrogen receptor

PR:

Progesterone receptor

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Acknowledgments

This work was supported by the grant No. 175056 of the Ministry of Science and Technology of the Republic of Serbia. We wish to thank Prof. Dr. M. Susa for suggestions and help during this investigation. Also, we wish to thank Mrs. Jasna Popovic-Basic and Mrs. Marijana Topalovic for excellent technical work during this investigation and Mrs. Dusica Gavrilovic for extensive statistical analyses.

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Authors and Affiliations

  1. Department of Radiation Oncology and Diagnostics, Institute of Oncology and Radiology of Serbia, Belgrade, Serbia

    Sandra Radenkovic & Zorica Milosevic

  2. Department of Experimental Oncology, Institute of Oncology and Radiology of Serbia, Belgrade, Serbia

    Gordana Konjevic

  3. Department of Pathology, Institute of Oncology and Radiology of Serbia, Belgrade, Serbia

    Katarina Karadzic

  4. Surgical Oncology Clinic, Institute of Oncology and Radiology of Serbia, Belgrade, Serbia

    Marko Buta

  5. School of Medicine, University of Belgrade, Belgrade, Serbia

    Zorica Milosevic, Gordana Konjevic, Branislav Rovcanin & Kristina Gopcevic

  6. Faculty of Medical Sciences, University of Kragujevac, P.BOX 124, 34 000, Kragujevac, Serbia

    Vladimir Jurisic

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  1. Sandra Radenkovic
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  2. Zorica Milosevic
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  7. Kristina Gopcevic
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Correspondence to Sandra Radenkovic or Vladimir Jurisic.

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Radenkovic, S., Milosevic, Z., Konjevic, G. et al. Lactate dehydrogenase, Catalase, and Superoxide dismutase in Tumor Tissue of Breast Cancer Patients in Respect to Mammographic Findings. Cell Biochem Biophys 66, 287–295 (2013). https://doi.org/10.1007/s12013-012-9482-7

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  • DOI: https://doi.org/10.1007/s12013-012-9482-7

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