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Effects of Nano-copper Oxide on Antioxidant Function of Copper-Deficient Kazakh Sheep

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Abstract

Kazakh sheep are vital to the production system of the Barkol prairie. The purpose of this study was to determine the effect of nano-copper oxide (Nano-Cu2O) on the antioxidant system of Cu-deficient Kazakh sheep in the Barkol prairie in Xinjiang, China. We analyzed mineral contents in soil, forage, and animal tissues. Blood parameters were also measured at the same time. The results showed that compared with healthy grassland, the Cu content in the soil and forage in Cu-deficient pastures was significantly lower than that in healthy grassland (P < 0.01). The Cu content in the blood, wool, and liver of Cu-deficient Kazakh sheep was significantly lower than that of healthy animals (P < 0.01). After Kazakh sheep were supplemented with Nano-Cu2O or CuSO4, the blood Cu concentration increased significantly (P < 0.01). From the 5th day, the Cu content of the Nano-Cu2O group was significantly higher than that of the CuSO4 group. The levels of hemoglobin (Hb), erythrocyte count (RBC), and packed cell volume (PCV) in the two experimental groups were significantly higher than those in Cu-deficient Kazakh sheep (P < 0.01). Compared with Cu-deficient Kazakh sheep, the serum ceruloplasmin (Cp) level of the two experimental groups increased significantly (P < 0.01), while the serum lactate dehydrogenase (LDH), alkaline phosphatase (AKP), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) decreased significantly (P < 0.01). Compared with Cu-deficient Kazakh sheep, the activities of serum superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), catalase (CAT), and total antioxidant capacity (T-AOC) in Nano-Cu2O and CuSO4 groups increased significantly (P < 0.01), while the level of serum malondialdehyde (MDA) decreased significantly (P < 0.01). Therefore, Nano-Cu2O could not only significantly increase the Cu content in the blood of Cu-deficient Kazakh sheep, but also greatly improve the antioxidant capacity.

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Data Availability

Data are available on request due to restriction privacy. The data presented in this study are available on request from the corresponding author. The data are not publicly available due to this paper is part of a series of studies, and disclosure of data may influence the publication of subsequent papers.

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Funding

This study was supported by the Innovation and Development Supporting Plan Project of Key Industries in Southern Xinjiang (2021DB014), the National Natural Science Foundation of China (42171060), the National Natural Science Foundation of China “Research on the effective active molecular groups and neural mechanism of Qingyangshen against post-traumatic stress disorder based on the spectrum-effect relationship” (31760091), and the Guizhou Medical University-the State Key Laboratory of Utilization of Medicinal Plants (NO.FAMP201705K).

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  1. Qingxiong Yang and Ping Zhou contributed equally to this work.

Authors and Affiliations

  1. School of Karst Science, Guizhou Normal University/State Engineering Technology Institute for Karst Desertification Control, Guiyang, 550001, China

    Xiaoying Min & Qingxiong Yang

  2. State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, 832000, Xinjiang, China

    Ping Zhou

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Contributions

Xiaoying Min: Conceptualization, data curation, formal analysis, investigation, methodology, resources, software, supervision, validation, visualization, writing—original draft, writing—review and editing. Qingxiong Yang: Conceptualization, data curation, formal analysis, funding acquisition, investigation, methodology, project administration, resources, software, supervision, validation, visualization, writing—original draft, writing—review and editing. Ping Zhou: Conceptualization, data curation, funding acquisition, investigation, methodology, project administration, visualization, writing—review and editing. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Qingxiong Yang or Ping Zhou.

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The Kazakh sheep that were used in the study were cared for as outlined in the Guide for the Care and Use of Animals in Agricultural Research and Teaching Consortium. Sample collections from the Kazakh sheep were approved by Southwest University of Science and Technology in the People’s Republic of China, Institutional Animal Care and Use Committee (Project A00566). All authors confirm ethical responsibility.

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Min, X., Yang, Q. & Zhou, P. Effects of Nano-copper Oxide on Antioxidant Function of Copper-Deficient Kazakh Sheep. Biol Trace Elem Res 200, 3630–3637 (2022). https://doi.org/10.1007/s12011-021-02975-w

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