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The Effects of Oral Administration of Molybdenum Fertilizers on Immune Function of Nanjiang Brown Goat Grazing on Natural Pastures Contaminated by Mixed Heavy Metal

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Abstract

Mineral development and metal smelting seriously polluted the surrounding groundwater and soil, threatening human health through the food chain. To investigate the effects of different sources of molybdenum (Mo) fertilizers on immune function of Nanjiang brown goats grazing on natural pastures under compound pollutions, fertilizing experiment was carried out in Liangshan Yi Nationality Prefecture of the Western Sichuan Plateau, China. Eighteen square hectometers of polluted meadows were fenced and were randomly divided into three groups (3 replications/group and 2 hm2/replication). A total of 54 healthy Nanjiang brown goats with an average BW of 31.6 ± 1.5 kg (1 year old) were used to this 30-day test (18 goats per group). The goats from CON group, AM group, and PM group were orally supplemented with deionized water, 15 mg Mo/BW·d (ammonium molybdate tetrahydrate), and 15 mg Mo/BW·d (potassium molybdate), respectively. Compared to CON group, the serum Fe content of grazing animals from AM group and PM group was 10.05% and 3.45% higher (P < 0.05), and the serum Cu content of grazing animals from AM group and PM group was 69.05% and 67.86% lower, respectively (P < 0.05). Mo fertilization significantly increased the levels of blood Hb, RBC, and PCV, and the activities of serum SOD, GSH-Px, CAT, and Cp of grazing goats (P < 0.05), and also extremely decreased the MDA content of experimental goats fed Mo compared to the control goats (P < 0.05). Compared to CON group, the activities of serum IgG, IgA, IgM, IL-2, and TNF-α of grazing animals from AM group and PM group were significantly increased (P < 0.05), and the levels of serum IL-6 and IL-1β of grazing goats from AM group and PM group were extremely decreased (P < 0.05). In summary, oral Mo fertilizers can alter the contents of serum mineral elements, reduce oxidative stress, improve immune function, and relieve the toxic damage of goats grazing on contaminated natural grasslands.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

The protocol for the present experiment was approved by the Institutional Animal Care and Use Committee of Southwest University of Science and Technology (SWUST20210136).

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Funding

This work was supported by the Innovation and Development Supporting Plan Project of Key Industries in Southern Xinjiang, China (2021DB014).

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Authors and Affiliations

  1. College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China

    Yuanfeng Li, Hongwei Liu, Jian He, Xiaoyun Shen & Yachao Wang

  2. Swine Research Institute, Tie Qi Li Shi Group Co., Mianyang, 621006, China

    Yuanfeng Li

  3. World Bank Poverty Alleviation Project Office in Guizhou, Southwest China, Guiyang, 550004, China

    Xiaoyun Shen

  4. State Key Laboratory of Sheep Genetic Improvement and Healthy Production, Xinjiang Academy of Agricultural and Reclamation Sciences, Shihezi, 832000, China

    Xiaoyun Shen

  5. School of Materials and Architectural Engineering, Guizhou Normal University, Guiyang, 550025, China

    Kui Zhao

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  1. Yuanfeng Li
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  2. Hongwei Liu
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  3. Jian He
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  4. Xiaoyun Shen
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Contributions

Yuanfeng Li: conceptualization, methodology, preparation, investigation, software, data curation, formal analysis, writing—original draft. Hongwei Liu: preparation, investigation, software, data curation, formal analysis, writing—original draft. Jian He: supervision, conceptualization, methodology, visualization, investigation, formal analysis, resources, writing—reviewing and editing. Xiaoyun Shen: supervision, conceptualization, methodology, investigation, project administration, funding acquisition. Kui Zhao: data curation, writing—reviewing and editing. Yachao Wang: validation, investigation, data curation.

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Correspondence to Jian He or Xiaoyun Shen.

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Yuanfeng Li and Hongwei Liu are equal contributors and co-first authors.

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Li, Y., Liu, H., He, J. et al. The Effects of Oral Administration of Molybdenum Fertilizers on Immune Function of Nanjiang Brown Goat Grazing on Natural Pastures Contaminated by Mixed Heavy Metal. Biol Trace Elem Res 200, 2750–2757 (2022). https://doi.org/10.1007/s12011-021-02901-0

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