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Effects of Supplemental Chromium Nanoparticles on IFN-γ expression of Heat Stress Broilers

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Abstract

The aim of present study was to investigate the beneficial effect of chromium (III) picolinate (CrPic) and chromium (III) picolinate nanoparticles (NCrPic) addition on growth performance, stress-related hormonal changes, and serum levels of various immunity biomarkers, as well as the gene expression of IFN-γ in broilers exposed to heat stress conditions. Treatments included T1 which received the basal diet with no feed additive; T2 exposed to heat stress; T3, T4, and T5 containing 500, 1000, and 1500 ppb CrPic; as well as T6, T7, and T8 containing 500, 1000, and 1500 ppb NCrPic, respectively. After 2 weeks from CrPic and NCrPic supplementation, IFN-γ mRNA expression was assayed using the RT-PCR technique. The results showed that the lower body weight, daily weight gain, daily feed intake by heat stress, and the feed conversion ratio were recovered remarkably by CrPic and NCrPic supplements. The stress-elevated levels of cortisol and immunoglobulin were reduced significantly using CrPic and NCrPic supplementation (P ≤ 0.05). The gene expression profile showed that the upregulated expression of IFN-γ was regulated by the addition of CrPic and NCrPic, in particular, to the diet; however, a full downregulation of IFN-γ expression was observed after week 2 of NCrPic supplementation. In conclusion, the results indicated that nanoparticle supplementation could be effective in reducing heat stress–induced detrimental alterations, thereby attributing to substantial changes to the immune system, including IFN-γ expression.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

References

  1. Sohail MU, Hume ME, Byrd JA, Nisbet DJ, Ijaz A, Sohail A, Shabbir MZ, Rehman H (2012) Effect of supplementation of prebiotic mannan-oligosaccharides and probiotic mixture on growth performance of broilers subjected to chronic heat stress. Poult Sci 91:2235–2240. https://doi.org/10.3382/ps.2012-02182

    Article  CAS  PubMed  Google Scholar 

  2. Shabana N, Kuldeep D, Mani S, Ruchi T, Jeon G, Laudadio V, Tufarelli V (2014) Modes of action and beneficial applications of chromium in poultry nutrition, production and health: a review. Int J Pharmacol 10(7):357–367. https://doi.org/10.3923/ijp.2014.357.367

    Article  CAS  Google Scholar 

  3. Farag MR, Alagawany M, El-Hack MEA, Muhammad A, Ayasan T, Kuldeep D, Kumaragurubaran K (2017) Role of chromium in poultry nutrition and health: beneficial applications and toxic effects. Int J Pharmacol 13(7):907–915. https://doi.org/10.3923/ijp.2017.907.915

    Article  CAS  Google Scholar 

  4. Saracila M, Panaite T, Tabuc C, Soica C, Untea A, Ayasan T, Criste RD (2020) Dietary ascorbic acid and chromium supplementation for broilers reared under thermoneutral conditions vs. high heat stress. Sci Pap Anim Sci Ser 73(1):41–47

    Google Scholar 

  5. Keshri A, Roy D, Kumar V, Kumar M, Kushwaha R, Vaswani S, Choudhury S (2019) Effect of chromium supplementation on rhythmic alterations in growth performance and nutrient utilization of growing cattle during heat stress. Biol Rhythm Res:1–9. https://doi.org/10.1080/09291016.2019.1616143

  6. Kargar S, Habibi Z, Karimi-Dehkordi S (2019) Grain source and chromium supplementation: effects on feed intake, meal and rumination patterns, and growth performance in Holstein dairy calves. Animal 13(6):1173–1179. https://doi.org/10.1017/S1751731118002793

    Article  CAS  PubMed  Google Scholar 

  7. Bahrami A, Moeini MM, Ghazi SH, Targhibi MR (2012) The effect of different levels of organic and inorganic chromium supplementation on immune function of broiler chicken under heat-stress conditions. J Appl Poult Res 21(2):209–215. https://doi.org/10.3382/japr.2010-00275

    Article  CAS  Google Scholar 

  8. Wang S, Wang J, Liu Y, Li H, Wang Q, Huang Z, Shi P (2019) Trivalent chromium supplementation ameliorates oleic acid-induced hepatic steatosis in mice. Biol Trace Elem Res 187(1):192–201. https://doi.org/10.1007/s12011-018-1368-0

    Article  CAS  PubMed  Google Scholar 

  9. Bhagat J, Ahmed KA, Tyagi P, Saxena M, Saxena VK (2008) Effects of supplemental chromium on interferon-gamma (IFN-γ) mRNA expression in response to Newcastle disease vaccine in broiler chicken. Res Vet Sci 85:46–51. https://doi.org/10.1016/j.rvsc.2007.08.003

    Article  CAS  PubMed  Google Scholar 

  10. Talukder AK, Rashid MB, Yousef MS, Kusama K, Shimizu T, Shimada M, Miyamoto A (2018) Oviduct epithelium induces interferon-tau in bovine day-4 embryos, which generates an anti-inflammatory response in immune cells. Sci Rep 8(1):1–13. https://doi.org/10.1038/s41598-018-26224-8

    Article  CAS  Google Scholar 

  11. Barjesteh N, Taha-Abdelaziz K, Kulkarni RR, Sharif S (2019) Innate antiviral responses are induced by TLR3 and TLR4 ligands in chicken tracheal epithelial cells: Communication between epithelial cells and macrophages. Virology 534:132–142. https://doi.org/10.1016/j.virol.2019.06.003

    Article  CAS  PubMed  Google Scholar 

  12. Lin YC, Huang JT, Li MZ, Cheng CY, Lien TF (2015) Effects of supplemental nanoparticle trivalent chromium on the nutrient utilization, growth performance and serum traits of broilers. J Anim Physiol Anim Nutr 99(1):59–65. https://doi.org/10.1111/jpn.12215

    Article  CAS  Google Scholar 

  13. Lien TF, Yeh HS, Lu FY, Fu CM (2009) Nanoparticles of chromium picolinate enhance chromium digestibility and absorption. J Sci Food Agric 89(7):1164–1167. https://doi.org/10.1002/jsfa.3569

    Article  CAS  Google Scholar 

  14. Aviagen R (2009) Ross broiler management manual, vol 2014, pp 350–364 http://pt.aviagen.com/assets/Tech_Center/Ross_Broiler/Ross_Broiler_Manual_9

    Google Scholar 

  15. Abudabos AM, Ali MH, Nassan MA, Saleh AA (2019) Ameliorative effect of Bacillus subtilis on growth performance and intestinal architecture in broiler infected with Salmonella. Animals 9(4):190. https://doi.org/10.3390/ani9040190

    Article  PubMed Central  Google Scholar 

  16. Martin JE, McKeegan DE, Sparrey J, Sandilands V (2017) Evaluation of the potential killing performance of novel percussive and cervical dislocation tools in chicken cadavers. Br Poult Sci 58(3):216–223. https://doi.org/10.1080/00071668.2017.1280724

    Article  CAS  PubMed  Google Scholar 

  17. Huang C, Liang D, Tatomer DC, Wilusz JE (2018) A length-dependent evolutionarily conserved pathway controls nuclear export of circular RNAs. Genes Dev 32(9-10):639–644. https://doi.org/10.1101/gad.314856.118

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  18. Ingerslev LR, Donkin I, Fabre O, Versteyhe S, Mechta M, Pattamaprapanont P, Barrès R (2018) Endurance training remodels sperm-borne small RNA expression and methylation at neurological gene hotspots. Clin Epigenetics 10(1):12. https://doi.org/10.1186/s13148-018-0446-7

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Naghieh A, Toghyani M, Gheisari AA, Egbal Saeed S, Mirzazade H (2010) Effect of different sources of chromium on performance and immune responses of broiler chicks. J Anim Vet Adv 9(2):354–358. https://doi.org/10.3923/javaa.2010.354.358

    Article  CAS  Google Scholar 

  20. Sahin N, Hayirli A, Orhan C, Tuzcu M, Komorowski JR, Sahin K (2018) Effects of the supplemental chromium form on performance and metabolic profile in laying hens exposed to heat stress. Poult Sci 97(4):1298–1305. https://doi.org/10.3382/ps/pex435

    Article  CAS  PubMed  Google Scholar 

  21. Lu L, Zhao LL, Dong SY, Liao XD, Dong XY, Zhang LY, Luo XG (2019) Dietary supplementation of organic or inorganic chromium modulates the immune responses of broilers vaccinated with Avian Influenza virus vaccine. Animal 13(5):983–991. https://doi.org/10.1017/S1751731118002379

    Article  CAS  PubMed  Google Scholar 

  22. Arif M, Hussain I, Mahmood MA, El-Hack A, Mohamed E, Swelum AA, Komany A (2019) Effect of varying levels of chromium propionate on growth performance and blood biochemistry of broilers. Animals 9(11):935. https://doi.org/10.3390/ani9110935

    Article  PubMed Central  Google Scholar 

  23. Ognik K, Stępniowska A, Kozłowski K (2017) The effect of administration of silver nanoparticles to broiler chickens on estimated intestinal absorption of iron, calcium, and potassium. Livest Sci 200:40–45. https://doi.org/10.1016/j.livsci.2017.04.002

    Article  Google Scholar 

  24. Song Y, Shi Y, Zhang L, Hu H, Zhang C, Yin M, Sun K (2019) Synthesis of CSK-DEX-PLGA nanoparticles for the oral delivery of exenatide to improve its mucus penetration and intestinal absorption. Mol Pharm 16(2):518–532. https://doi.org/10.1021/acs.molpharmaceut.8b00809

    Article  CAS  PubMed  Google Scholar 

  25. Talegaonkar S, Bhattacharyya A (2019) Potential of lipid nanoparticles (SLNs and NLCs) in enhancing oral bioavailability of drugs with poor intestinal permeability. AAPS PharmSciTech 20(3):121. https://doi.org/10.1208/s12249-019-1337-8

    Article  CAS  PubMed  Google Scholar 

  26. Valera M, Casasola R, Gutiérrez O, Sánchez-Chiprés DR, Mireles S (2021) Effects of supplementation with a novel organic chromium product on metabolic and physiological indicators of broilers. J Anim Health Prod 9(1):13–21. https://doi.org/10.17582/journal.jahp/2021/9.1.13.21

    Article  Google Scholar 

  27. Ebrahimzadeh SK, Farhoomand P, Noori K (2012) Immune response of broiler chickens fed diets supplemented with different level of chromium methionine under heat stress conditions. Asian Australas J Anim Sci 25(2):256–260. https://doi.org/10.5713/ajas.2011.11217

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  28. Tahir SK, Yousaf MS, Rashid MA, Khan AF, Ahmad S, Zaneb H, Rehman H (2019) Supplemental chromium-loaded chitosan nanoparticles affect growth, serum metabolites and intestinal histology in broilers. S Afr J Anim Sci 49(6):1072–1082. https://doi.org/10.4314/sajas.v49i6.11

    Article  CAS  Google Scholar 

  29. Rifaai RA, Abd El Baky FAZF (2017) Histological and immunohistochemical study on the effect of constant light exposure on T lymphocyte subsets in the thymus and lymph node of male albino rats. Egypt J Histolog 40(1):116–128. https://doi.org/10.21608/EJH.2017.3697

    Article  Google Scholar 

  30. Odinokov D, Hamblin MR (2018) Aging of lymphoid organs: Can photobiomodulation reverse age-associated thymic involution via stimulation of extrapineal melatonin synthesis and bone marrow stem cells? J Biophotonics 11(8):e201700282. https://doi.org/10.1002/jbio.201700282

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. ThyagaRajan S, Hima L, Pratap UP, Priyanka HP, Vasantharekha R (2019) Estrogen-induced neuroimmunomodulation as facilitator of and barrier to reproductive aging in brain and lymphoid organs. J Chem Neuroanat 95:6–12. https://doi.org/10.1016/j.jchemneu.2018.02.008

    Article  CAS  PubMed  Google Scholar 

  32. Moeini MM, Bahrami A, Ghazi S, Targhibi MR (2011) The effect of different levels of organic and inorganic chromium supplementation on production performance, carcass traits and some blood parameters of broiler chicken under heat stress condition. Biol Trace Elem Res 144(1-3):715–724. https://doi.org/10.1007/s12011-011-9116-8

    Article  CAS  PubMed  Google Scholar 

  33. Huang YL, Luo QH, Xiao F, Lin X, Spears JW (2020) Research Note: Responses of growth performance, immune traits, and small intestinal morphology to dietary supplementation of chromium propionate in heat-stressed broilers. Poult Sci 99(10):5070–5073. https://doi.org/10.1016/j.psj.2020.07.005

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Wasti S, Sah N, Mishra B (2020) Impact of heat stress on poultry health and performances, and potential mitigation strategies. Animals 10(8):1266. https://doi.org/10.3390/ani10081266

    Article  PubMed Central  Google Scholar 

  35. Sharma P, Pande VV, Moyle TS, McWhorter AR, Chousalkar KK (2017) Correlating bacterial shedding with fecal corticosterone levels and serological responses from layer hens experimentally infected with Salmonella Typhimurium. Vet Res 48(1):5. https://doi.org/10.1186/s13567-017-0414-9

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  36. Hirakawa R, Nurjanah S, Furukawa K, Murai A, Kikusato M, Nochi T, Toyomizu M (2020) Heat stress causes immune abnormalities via massive damage to effect proliferation and differentiation of lymphocytes in broiler chickens. Front Vet Sci 7:46. https://doi.org/10.3389/fvets.2020.00046

    Article  PubMed  PubMed Central  Google Scholar 

  37. Kojima M, Oji S, Tanaka S, Izaki S, Hashimoto B, Fukaura H, Nomura K (2020) Tacrolimus is effective for neuromyelitis optica spectrum disorders with or without anti-AQP4 antibody. Mult Scler Relat Disord 39:101907. https://doi.org/10.1016/j.msard.2019.101907

    Article  Google Scholar 

  38. Shen W, Poliquin S, Macdonald RL, Dong M, Kang JQ (2020) Endoplasmic reticulum stress increases inflammatory cytokines in an epilepsy mouse model Gabrg2+/Q390X knockin: a link between genetic and acquired epilepsy? Epilepsia 61(10):2301–2312. https://doi.org/10.1111/epi.16670

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  39. Podojil JR, Glaser AP, Baker D, Courtois ET, Fantini D, Yu Y, Meeks JJ (2020) Antibody targeting of B7-H4 enhances the immune response in urothelial carcinoma. OncoImmunology 9(1):1744897. https://doi.org/10.1080/2162402X.2020.1744897

    Article  PubMed  PubMed Central  Google Scholar 

  40. Mirfendereski E, Jahanian R (2015) Effects of dietary organic chromium and vitamin C supplementation on performance, immune responses, blood metabolites, and stress status of laying hens subjected to high stocking density. Poult Sci 94(2):281–288. https://doi.org/10.3382/ps/peu074

    Article  CAS  PubMed  Google Scholar 

  41. Acharya N, Madi A, Zhang H, Klapholz M, Escobar G, Dulberg S, Anderson AC (2020) Endogenous glucocorticoid signaling regulates CD8+ T cell differentiation and development of dysfunction in the tumor microenvironment. Immunity 53(3):658–671. https://doi.org/10.1016/j.immuni.2020.08.005

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  42. Spears JW, Lloyd KE, Siciliano P, Pratt-Phillips S, Goertzen EW, McLeod SJ, Rounds W (2020) Chromium propionate increases insulin sensitivity in horses following oral and intravenous carbohydrate administration. J Anim Sci 98(4):skaa095. https://doi.org/10.1093/jas/skaa095

    Article  PubMed  PubMed Central  Google Scholar 

  43. Song H, Park H, Kim YS, Kim KD, Lee HK, Cho DH, Yang JW, Hur DY (2011) L-kynurenine-induced apoptosis in human NK cells is mediated by reactive oxygen species. Int Immunopharmacol 11:932–938. https://doi.org/10.1016/j.intimp.2011.02.005

    Article  CAS  PubMed  Google Scholar 

  44. Landman GW, Bilo HJ, Houweling ST, Kleefstra N (2014) Chromium does not belong in the diabetes treatment arsenal: current evidence and future perspectives. World J Diabetes 5(2):160–164. https://doi.org/10.4239/wjd.v5.i2.160

    Article  PubMed  PubMed Central  Google Scholar 

  45. Hassan S, Hassan FU, Rehman MSU (2020) Nano-particles of trace minerals in poultry nutrition: potential applications and future prospects. Biol Trace Elem Res 195(2):591–612. https://doi.org/10.1007/s12011-019-01862-9

    Article  CAS  PubMed  Google Scholar 

  46. Lee J, Hosseindoust A, Kim M, Kim K, Choi Y, Lee S, Chae B (2020) Biological evaluation of hot-melt extruded nano-selenium and the role of selenium on the expression profiles of selenium-dependent antioxidant enzymes in chickens. Biol Trace Elem Res 194(2):536–544. https://doi.org/10.1007/s12011-019-01801-8

    Article  CAS  PubMed  Google Scholar 

  47. Stępniowska A, Tutaj K, Drażbo A, Kozłowski K, Ognik K, Jankowski J (2020) Estimated intestinal absorption of phosphorus and its deposition in chosen tissues, bones and feathers of chickens receiving chromium picolinate or chromium nanoparticles in diet. PLoS One 15(11):e0242820. https://doi.org/10.1371/journal.pone.0242820

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Authors and Affiliations

  1. Department of Animal Science, Science and Research Branch, Islamic Azad University, Tehran, Iran

    Omid Hamidi, Mohammad Chamani & Ali Asghar Sadeghi

  2. Faculty of Veterinary Medicine, Urmia Branch, Islamic Azad University, Urmia, Iran

    Hassan Ghahri

  3. Faculty of Pharmacy, Urmia University of Medical Sciences, Urmia, Iran

    Hassan Malekinejad

  4. Department of Animal Science, Agricultural Faculty, Ataturk University, 25240, Erzurum, Turkey

    Valiollah Palangi

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  1. Omid Hamidi
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  2. Mohammad Chamani
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  3. Hassan Ghahri
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  4. Ali Asghar Sadeghi
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Contributions

OH and MC contributed to the project idea, design and execution of the study. HG, AS, and HM were in charge of laboratory analyses. VP was responsible for scientific editing and finalizing the manuscript.

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Correspondence to Hassan Ghahri.

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Ethical protocol was approved by the experimental animal ethics committee of Islamic Azad University of Tehran Science and Research Branch, Tehran, Iran.

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The authors declare no competing interests.

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Hamidi, O., Chamani, M., Ghahri, H. et al. Effects of Supplemental Chromium Nanoparticles on IFN-γ expression of Heat Stress Broilers. Biol Trace Elem Res 200, 339–347 (2022). https://doi.org/10.1007/s12011-021-02634-0

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