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Excess Manganese-Induced Apoptosis in Chicken Cerebrums and Embryonic Neurocytes

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Abstract

There were many studies about the effect of excess manganese (Mn) on nervous system apoptosis; however, Mn-induced apoptosis in chicken cerebrums and embryonic neurocytes was unclear. The purpose of this study was to investigate the effect of excess Mn on chicken cerebrum and embryonic neurocyte apoptosis. Seven-day-old Hyline male chickens were fed either a commercial diet or three levels of manganese chloride (MnCl2)-added commercial diets containing 600-, 900-, and 1800-mg/kg-Mn diet, respectively. On the 30th, 60th, and 90th days, cerebrums were collected. Fertilized Hyline chicken eggs were hatched for 6–8 days and were selected. Embryonic neurocytes with 0, 0.5, 1, 1.5, 2, 2.5, and 3 mM Mn were collected and were cultured for 12, 24, 36, and 48 h, respectively. The following research contents were performed: superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) activities; tumor protein p53 (p53), B cell lymphoma-2 (Bcl-2), B cell lymphoma extra large (Bcl-x), Bcl-2-associated X protein (Bax), Bcl-2 homologous antagonist/killer (Bak), fas, and caspase-3 messenger RNA (mRNA) expression; and morphologic observation. The results indicated that excess Mn inhibited SOD and T-AOC activities; induced p53, Bax, Bak, fas, and caspase-3 mRNA expression; and inhibited Bcl-2 and Bcl-x mRNA expression in chicken cerebrums and embryonic neurocytes. There were dose-dependent manners on all the above factors at all the time points and time-dependent manners on SOD activity of 1800-mg/kg-Mn group, T-AOC activity, and apoptosis-related gene mRNA expression in all the treatment groups in chicken cerebrums. Excess Mn induced chicken cerebrum and embryonic neurocyte apoptosis.

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Authors and Affiliations

  1. College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Kun Zhang, Yihao Zhu, Xiaoyu Wang, Xin Zhao & Xiaohua Teng

  2. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Shu Li

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  1. Kun Zhang
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  2. Yihao Zhu
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  3. Xiaoyu Wang
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  4. Xin Zhao
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  6. Xiaohua Teng
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Corresponding authors

Correspondence to Shu Li or Xiaohua Teng.

Ethics declarations

All procedures used in this experiment were approved by the Northeast Agricultural University’s Institutional Animal Care and Use Committee under the approved protocol number SRM-06. Informed consent was obtained from all individual participants included in the study.

Funding

This work was supported by grants from Hualong Feed Research Collaboration (No. 860035) and the Natural Science Foundation of Heilongjiang Province of China (No. C201420).

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The authors declare that they have no conflict of interest.

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All authors have read the manuscript and have agreed to submit it in its current form for consideration for publication in Biological Trace Element Research.

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Zhang, K., Zhu, Y., Wang, X. et al. Excess Manganese-Induced Apoptosis in Chicken Cerebrums and Embryonic Neurocytes. Biol Trace Elem Res 180, 297–305 (2017). https://doi.org/10.1007/s12011-017-0992-4

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  • DOI: https://doi.org/10.1007/s12011-017-0992-4

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