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The Effect of Manganese-induced Cytotoxicity on mRNA Expressions of HSP27, HSP40, HSP60, HSP70 and HSP90 in Chicken Spleen Lymphocytes in Vitro

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Abstract

The purpose of this study was to investigate the effect of manganese (Mn)-induced cytotoxicity on heat shock proteins in chicken spleen lymphocytes. Lymphocytes were cultured in medium in the absence and presence of MnCl2 (2 × 10−4, 4 × 10−4, 6 × 10−4, 8 × 10−4, 10 × 10−4, and 12 × 10−4 mmol/L) for 12, 24, 36, and 48 h in vitro. Then, the mRNA levels of HSP27, HSP40, HSP60, HSP70, and HSP90 were examined by real-time quantitative PCR. The results showed that the mRNA levels of HSP27, HSP40, HSP60, HSP70, and HSP90 in all treatment groups at all time points, except mRNA levels of HSP27 at 48 h, had the same tendency. As manganese concentration increased, the mRNA expression of the heat shock proteins first increased and then decreased. In other words, we demonstrated that the mRNA expression of the heat shock proteins was induced at lower concentrations of manganese and was inhibited at higher concentrations. Mn had a dosage-dependent effect on HSP27, HSP40, HSP60, HSP70, and HSP90 mRNA expression in chicken spleen lymphocytes in vitro.

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Abbreviations

Mn:

Manganese

HSPs:

Heat shock proteins

qPCR:

Real-time quantitative PCR

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Acknowledgments

We express our sincere appreciation to all the participants of this study. This work was supported in part by a grant from the Doctoral Program Fund of Institutions of Higher Education of China (no. 2010RCB32), China.

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Authors and Affiliations

  1. College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Yihao Zhu, Xinxing Lu, Di Wu & Xiaohua Teng

  2. Supervision Institution of Veterinary Medicine and Feed in Heilongjiang Province, Harbin, 150001, People’s Republic of China

    Shaohe Cai

  3. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Shu Li

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  1. Yihao Zhu
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Zhu, Y., Lu, X., Wu, D. et al. The Effect of Manganese-induced Cytotoxicity on mRNA Expressions of HSP27, HSP40, HSP60, HSP70 and HSP90 in Chicken Spleen Lymphocytes in Vitro. Biol Trace Elem Res 156, 144–152 (2013). https://doi.org/10.1007/s12011-013-9817-2

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