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Toxicological Effect of Manganese on NF-κB/iNOS-COX-2 Signaling Pathway in Chicken Testes

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Abstract

Manganese (Mn) pollution can cause tissue and organ dysfunction and structural damage. The toxicity of Mn in poultry was reported, but inflammatory damage that Mn induced in the testicular tissue has not been reported. The aim of this study was to investigate the effect of Mn poisoning on NF-κB/iNOS-COX-2 signaling pathway in chicken testes. One hundred eighty Hyline male chickens at 7 days of age were fed either commercial diet or MnCl2-added commercial diet containing 600, 900, and 1800 mg/kg Mn for 30, 60, and 90 days, respectively. The messenger RNA (mRNA) expression of nuclear factor-κB (NF-κB), tumor necrosis factor-α (TNF-α), cyclooxygenase-2 (COX-2), and inducible nitric oxide synthase (iNOS), nitric oxide (NO) content, iNOS activity, and histopathology were examined in chicken testes. The results showed that excess Mn upregulated mRNA expression of NF-κB, COX-2, TNF-α, and iNOS, NO content, and iNOS activity at 60th and 90th day. Mn had a time-dependent effect on NF-κB and TNF-α mRNA expression. Mn had a dose- and time-dependent effect on NO content and iNOS activity. Mn exposure induced chicken testis histological changes in dose- and time-dependent manner. It indicated that Mn exposure resulted in inflammatory injury of chicken testis tissue through NF-κB/iNOS-COX-2 signaling pathway.

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Acknowledgments

The authors thank the Doctoral Program Foundation of Institutions of Institutions of Higher Education of China (No. 2010RCB32) and the Heilongjiang Province on the Natural Fund Project (41400172-4-14089).

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The authors declare no conflicts of interest.

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Authors and Affiliations

  1. College of Animal Science and Technology, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Ye Du, Yihao Zhu, Kun Zhang & Xiaohua Teng

  2. Heilongjiang Grassland and Forage Central Experimental Station, Harbin, 150069, People’s Republic of China

    Xiaojie Teng

  3. College of Veterinary Medicine, Northeast Agricultural University, Harbin, 150030, People’s Republic of China

    Shu Li

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  1. Ye Du
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  2. Yihao Zhu
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  3. Xiaojie Teng
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Du, Y., Zhu, Y., Teng, X. et al. Toxicological Effect of Manganese on NF-κB/iNOS-COX-2 Signaling Pathway in Chicken Testes. Biol Trace Elem Res 168, 227–234 (2015). https://doi.org/10.1007/s12011-015-0340-5

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