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Lanthanum(III) Impacts on Metallothionein MTT1 and MTT2 from Tetrahymena thermophila

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Abstract

Metallothionein MTT1 and MTT2 from Tetrahymena thermophila are sulfydryl-rich proteins that can bind to and are inducible by heavy metals such as mercury, cadmium, zinc, and copper. However, little is known about the induction and binding of T. thermophila metallothionein by trivalent metals. In this study, we found that 10–80 μM La3+ can promote Tetrahymena cells proliferation, and fluorescence spectrum analysis showed that La3+ can enter T. thermophila cells. Real-time quantitative polymerase chain reaction showed La3+ induced the expression of MTT1 and MTT2. Furthermore, Fluorescence analysis indicated La3+ bind to MTT1 and MTT2. These results implied that La3+ could interact with MTT1 and MTT2 via aspartic or glutamic acid oxygen atoms.

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Acknowledgements

This work was supported by grants from the National Natural Scientific Foundation of China (30770295 and 31072000), the Natural Scientific Foundation of Shanxi Province (No. 2008011063), and Program for the Top Young Academic Leaders of Higher Learning Institutions of Shanxi to W. Wang. Preliminary sequence data were obtained from http://www.ciliate.org.

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Authors and Affiliations

  1. Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Institute of Biotechnology, Shanxi University, 92 Wucheng Rd, Taiyuan, 030006, People’s Republic of China

    Qinglu Wang, Jing Xu, Yajing Zhu, Baofeng Chai, Aihua Liang & Wei Wang

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  1. Qinglu Wang
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  2. Jing Xu
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  3. Yajing Zhu
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  4. Baofeng Chai
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  6. Wei Wang
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Correspondence to Wei Wang.

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Wang, Q., Xu, J., Zhu, Y. et al. Lanthanum(III) Impacts on Metallothionein MTT1 and MTT2 from Tetrahymena thermophila . Biol Trace Elem Res 143, 1808–1818 (2011). https://doi.org/10.1007/s12011-011-9004-2

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