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Collision/Reaction Cell ICP-MS with Shielded Torch and Sector Field ICP-MS for the Simultaneous Determination of Selenium Isotopes in Biological Matrices

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Abstract

The determinations of selenium isotopes in biological samples were performed using both inductively coupled plasma collision/reaction cell quadruple mass spectrometer (CRC-ICP-QMS) and inductively coupled plasma sector field mass spectrometers (SF-ICP-MS). To significantly decrease the argon-based interferences at m/z 74 (36Ar38Ar), 76 (38Ar38Ar, 40Ar36Ar), 78 (38Ar40Ar), and 80 (40Ar40Ar), the gas-flow rates of a helium and hydrogen mixture used in the collision cell were optimized to 1.0 mL/min H2 and 3.5 mL/min He. Under the optimized condition, the precisions for natural selenium isotope ratio measurements of both instruments were evaluated and compared using 100 ppb Se standard solution. A modified external calibration quantification method was applied for the simultaneous determination of clinically used enriched selinocompounds (77Se-selenate, 82Se-selenite, 76Se-methylseleninic acidIV, 78Se-methylselenonic acidVI) and to examine their fate in rat organs (liver, kidney, and lung).

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Authors and Affiliations

  1. Central Laboratories Unit, Qatar University, P.O. Box 2713, Doha, Qatar

    Khalid A. Al-Saad & Mohammed A. Amr

  2. Nuclear Physics Department, NRC, Atomic Energy, Cairo, 13759, Egypt

    Mohammed A. Amr & Abdulfattah I. Helal

Authors
  1. Khalid A. Al-Saad
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  2. Mohammed A. Amr
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  3. Abdulfattah I. Helal
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Correspondence to Mohammed A. Amr.

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Al-Saad, K.A., Amr, M.A. & Helal, A.I. Collision/Reaction Cell ICP-MS with Shielded Torch and Sector Field ICP-MS for the Simultaneous Determination of Selenium Isotopes in Biological Matrices. Biol Trace Elem Res 140, 103–113 (2011). https://doi.org/10.1007/s12011-010-8677-2

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  • DOI: https://doi.org/10.1007/s12011-010-8677-2

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