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Biochemical Characterization of Thermostable and Detergent-Tolerant β-Agarase, PdAgaC, from Persicobacter sp. CCB-QB2

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Abstract

Persicobacter sp. CCB-QB2 belonging to the family Flammeovirga is an agarolytic bacterium and exhibits a diauxic growth in the presence of tryptone and agarose. A glycoside hydrolase (GH) 16 β-agarase, PdAgaC, was identified in the genome of the bacterium and was highly expressed during the second growth phase, indicating the agarase may play an important role in the diauxic growth. In this study, the catalytic domain of PdAgaC (PdAgaCgh) was cloned and characterized. PdAgaCgh showed thermostability at 50 °C and tolerance towards several detergents. In addition, the activity of PdAgaCgh after incubation with 0.1% of SDS and Triton X-100 increased approximately 1.2-fold. On the other hand, PdAgaCgh was sensitive to Fe2+, Ni2+, and Cu2+. The Km and Vmax of PdAgaCgh were 5.15 mg/ml and 2.9 × 103 U/mg, respectively. Interestingly, although the major hydrolytic product was neoagarobiose (NA2), monomeric sugar was also detected by thin-layer chromatographic analysis.

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Acknowledgements

G. Furusawa gratefully acknowledges the post-doctoral fellowships granted by Universiti Sains Malaysia.

Funding

This project was financially supported by the Research University (RU) mangrove project grant (1001/PCCB/870009).

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  1. Centre for Chemical Biology, Universiti Sains Malaysia, 11900, Penang, Malaysia

    Nur Fazli Hafizah, Aik-Hong Teh & Go Furusawa

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  1. Nur Fazli Hafizah
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  2. Aik-Hong Teh
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Correspondence to Go Furusawa.

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The authors declare that they have no conflict of interest. All authors have contributed to the work, and they have agreed to submit the manuscript.

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Hafizah, N.F., Teh, AH. & Furusawa, G. Biochemical Characterization of Thermostable and Detergent-Tolerant β-Agarase, PdAgaC, from Persicobacter sp. CCB-QB2. Appl Biochem Biotechnol 187, 770–781 (2019). https://doi.org/10.1007/s12010-018-2849-5

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