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Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production

  • IBS 2012 (15th International Biotechnology Symposium)
  • Published:
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Abstract

Candida rugosa lipase and Ryzopus oryzae lipase were simultaneously immobilized on silica gel following enzyme pretreatment. The factors affecting the co-immobilization process, such as reaction time and enzyme ratio, were investigated. Biodiesel was then produced by using the co-immobilized enzyme matrix. A batch system was employed with stepwise methanol feeding, and the continuous process involved a packed-bed reactor. Under optimal immobilization conditions, the activity was approximately 16,000 U/g·matrix. When co-immobilized enzyme was used with optimized stepwise methanol feeding, conversion of biodiesel reached about 99% at 3 h and was maintained at a level of over 90% for about 30 reuses.

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Authors and Affiliations

  1. Department of Chemical and Biological Engineering, Korea University, 5-1, Anam-dong, Sungbuk-gu, Seoul, 136-701, Korea

    Jong Ho Lee, Sung Bong Kim, Hah Young Yoo, Ja Hyun Lee & Seung Wook Kim

  2. College of Life Science and Biotechnology, Korea University, 5-1, Anam-dong, Sungbuk-gu, Seoul, 136-701, Korea

    Sung Ok Han

  3. Department of Chemical Engineering, Kwangwoon University, 447-1, Wolgye-dong, Nowon-gu, Seoul, 139-701, Korea

    Chulhwan Park

Authors
  1. Jong Ho Lee
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  2. Sung Bong Kim
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  3. Hah Young Yoo
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  4. Ja Hyun Lee
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  5. Sung Ok Han
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  6. Chulhwan Park
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  7. Seung Wook Kim
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Correspondence to Seung Wook Kim.

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Lee, J.H., Kim, S.B., Yoo, H.Y. et al. Co-immobilization of Candida rugosa and Rhyzopus oryzae lipases and biodiesel production. Korean J. Chem. Eng. 30, 1335–1338 (2013). https://doi.org/10.1007/s11814-013-0058-z

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  • DOI: https://doi.org/10.1007/s11814-013-0058-z

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