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Production of Mannitol from a High Concentration of Glucose by Candida parapsilosis SK26.001

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Abstract

A novel strain, SK26.001, which can produce mannitol from a high concentration of glucose without the addition of fructose, was isolated from sugarcane juice. This strain was identified as Candida parapsilosis based on 18S ribosomal RNA (rRNA) sequence analysis and the morphological and physiological-biochemical characteristics of the strain. Under optimized fermentation conditions, the mannitol concentration in shake flasks reached 68.5 g/L. When batch fermentation was performed, the fed glucose was completely consumed after 72 h, resulting in a final mannitol concentration of 80.3 g/L. Fed-batch fermentation was then performed with glucose feed. During the fed-batch process, ammonia water was added to maintain the pH at 4.0. The mannitol concentration in the fermenter reached 97.1 g/L after 120 h, with a total glucose consumption of 284 g/L.

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Acknowledgments

This work was financially supported by the 863 project of China (no. 2013AA102102) and the Fundamental Research Funds for the Central Universities (SKLF-ZZA-201509).

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Authors and Affiliations

  1. State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, 214122, China

    Qing Meng, Tao Zhang, Wenting Wei, Wanmeng Mu & Ming Miao

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  1. Qing Meng
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  2. Tao Zhang
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  3. Wenting Wei
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Correspondence to Tao Zhang.

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Meng, Q., Zhang, T., Wei, W. et al. Production of Mannitol from a High Concentration of Glucose by Candida parapsilosis SK26.001. Appl Biochem Biotechnol 181, 391–406 (2017). https://doi.org/10.1007/s12010-016-2219-0

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  • DOI: https://doi.org/10.1007/s12010-016-2219-0

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