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Ca2+ and Cu2+ supplementation increases mannitol production by Candida magnoliae

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Abstract

Supplementation with CaCl2·2H2O (50 mg l−1) or CuSO4·5H2O (10 mg l−1) improved mannitol production by Candida magnoliae by 14.5 and 18.6% (25 and 32 g/L), respectively. When used in combination, they acted synergistically: Ca2+ decreased the intracellular concentration of mannitol 30%, whereas Cu2+ increased the intracellular activity of mannitol dehydrogenase 1.6-times more than control. Ca2+ probably works by altering the permeability of cells to mannitol, whereas, Cu2+ increases the activity of an enzyme responsible for mannitol biosynthesis.

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Acknowledgment

This paper was supported by Konkuk University in 2006.

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Authors and Affiliations

  1. Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, Korea, 143-701

    Jung-Kul Lee, Hee-Yeol Song & In-Won Kim

  2. Department of Bioscience and Biotechnology, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, Korea, 143-701

    Deok-Kun Oh

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  1. Jung-Kul Lee
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  2. Deok-Kun Oh
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  3. Hee-Yeol Song
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  4. In-Won Kim
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Correspondence to In-Won Kim.

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Lee, JK., Oh, DK., Song, HY. et al. Ca2+ and Cu2+ supplementation increases mannitol production by Candida magnoliae . Biotechnol Lett 29, 291–294 (2007). https://doi.org/10.1007/s10529-006-9230-4

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  • DOI: https://doi.org/10.1007/s10529-006-9230-4

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