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Physicochemical Characterization of an Exopolysaccharide Produced by a Newly Isolated Weissella cibaria

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Abstract

A high molecular weight (3037 kDa) dextran-like polysaccharide was purified from the culture broth of a probiotic Weissella cibaria. Dry weight of the exopolysaccharides (EPS) obtained after ethanol precipitation was 6.35 g/L by using sucrose as carbon source and was further purified by phenol:chloroform:isoamyl alcohol (25:24:1) extraction and acetone precipitation to white powder with 52 % recovery. Rheological characterization revealed that it has a pseudoplastic behaviour and showed a temperature tolerance of up to 280 °C in thermogravimetric analysis. Fourier transform infrared (FTIR) spectra showed the characteristic peaks of polysaccharide such as hydroxyl group, α-pyranose, asymmetrical C–H stretching vibration of methyl group and carbonyl hydrogens of d-glucopyranose. The 1H nuclear magnetic resonance (NMR) spectrum showed resonances of hydrogen corresponding to the glucosyl residue, a repeat unit of the biopolymer. The cell viability analysis of normal L6 cells treated with the Weissella EPS showed that it is noncytotoxic even up to the 500-μg/mL level. A 0.2 % (w/v) supplementation of EPS in 2 % (w/v) wheat starch preparation could reduce the degree of syneresis up to 45 % and up to 75 % with 1 % EPS.

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Acknowledgments

The authors would like to acknowledge the XIIth five year plan Network project of CSIR, FUNHEALTH (CSC 0133) for the financial support. Chemical Sciences and Functional Materials Divisions in CSIR–NIIST are acknowledged for providing FTIR, NMR, TGA and rheological analyses facilities.

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Authors and Affiliations

  1. Biotechnology Division, National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Trivandrum, Thiruvananthapuram, 695 019, Kerala, India

    Dilna Sasidharan Vasanthakumari, Surya Harikumar, Divya Jayakumar Beena, Ashok Pandey & Kesavan Madhavan Nampoothiri

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  1. Dilna Sasidharan Vasanthakumari
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  2. Surya Harikumar
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  3. Divya Jayakumar Beena
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  4. Ashok Pandey
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  5. Kesavan Madhavan Nampoothiri
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Correspondence to Kesavan Madhavan Nampoothiri.

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Practical Application

The exopolysaccharide from Weissella cibaria was found to be of higher molecular weight, thermostable and pseudoplastic in nature. These properties account for its potential application in food industry as viscosifier, texturizer and emulsification agent.

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Supplementary Fig. 1

Standard calibration curve for GPC analysis. (GIF 156 kb)

High resolution image (TIFF 612 kb)

Supplementary Fig. 2

13C NMR spectra for EPS. (GIF 51 kb)

High resolution image (TIFF 17347 kb)

Supplementary Fig. 3

Prevention of syneresis in wheat starch (WS) by Weissella EPS at varying concentrations. (GIF 48 kb)

High resolution image (TIFF 16965 kb)

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Vasanthakumari, D.S., Harikumar, S., Beena, D.J. et al. Physicochemical Characterization of an Exopolysaccharide Produced by a Newly Isolated Weissella cibaria . Appl Biochem Biotechnol 176, 440–453 (2015). https://doi.org/10.1007/s12010-015-1586-2

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