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Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Production by a Moderate Halophile Yangia sp. ND199 Using Glycerol as a Carbon Source

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Abstract

Yangia sp. ND199, a moderate halophile isolated from mangrove soil sample in Vietnam, was found to accumulate poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from unrelated carbon sources in a medium with 4.5 % (w/v) NaCl. Cultivation with glycerol as carbon source and yeast extract as nitrogen source resulted in maximum cell dry weight of 5.7 g/l and PHBV content of 52.8 wt% (containing 2.9 mol% of 3HV) after 40 h. The 3HV content of the PHBV was the highest during initial stages of copolymer production and decreased with increase in the copolymer amount with time, but was not affected by changing the pH of the culture medium. Only homopolymer poly(3-hydroxybutyrate) was synthesized when monosodium glutamate was used as the nitrogen source. Fed-batch cultivation of Yangia sp. ND199 with glycerol and yeast extract gave PHBV content and productivity of 53.2 wt% and 0.44 g/l/h, respectively, which were reduced to 40.6 wt% and 0.25 g/l/h, respectively, with crude glycerol as carbon source. Both the copolymer content and productivity were improved to 56 wt% and 0.61 g/l/h, respectively, by using 1:1 mixture of crude glycerol and high fructose corn syrup. This is the first report of PHBV production by a wild-type halophilic bacterium using glycerol as carbon source.

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Acknowledgments

The authors are grateful to the National Foundation for Science and Technology Development (Grant no. 106.03-2010.64) and the Swedish Research Council through the Swedish Research Links programme for supporting this work.

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Authors and Affiliations

  1. Department of Microbiology and Biotechnology, Faculty of Biology, Hanoi National University of Education, 136 XuanThuy, CauGiay, Hanoi, Vietnam

    Doan Van-Thuoc, Tran Huu-Phong & Dang Minh-Khuong

  2. Department of Biotechnology, Center for Chemistry and Chemical Engineering, Lund University, P.O. Box 124, SE-221 00, Lund, Sweden

    Rajni Hatti-Kaul

Authors
  1. Doan Van-Thuoc
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  2. Tran Huu-Phong
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  3. Dang Minh-Khuong
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  4. Rajni Hatti-Kaul
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Correspondence to Rajni Hatti-Kaul.

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Van-Thuoc, D., Huu-Phong, T., Minh-Khuong, D. et al. Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Production by a Moderate Halophile Yangia sp. ND199 Using Glycerol as a Carbon Source. Appl Biochem Biotechnol 175, 3120–3132 (2015). https://doi.org/10.1007/s12010-015-1479-4

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  • DOI: https://doi.org/10.1007/s12010-015-1479-4

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