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Partial Characterization of Xylanase Produced by Caldicoprobacter algeriensis, a New Thermophilic Anaerobic Bacterium Isolated from an Algerian Hot Spring

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Abstract

To date, xylanases have expanded their use in many processing industries, such as pulp, paper, food, and textile. This study aimed the production and partial characterization of a thermostable xylanase from a novel thermophilic anaerobic bacterium Caldicoprobacter algeriensis strain TH7C1T isolated from a northeast hot spring in Algeria. The obtained results showed that C. algeriensis xylanase seems not to be correlated with the biomass growth profile whereas the maximum enzyme production (140.0 U/ml) was recorded in stationary phase (18 h). The temperature and pH for optimal activities were 70 °C and 11.0, respectively. The enzyme was found to be stable at 50, 60, 70, and 80 °C, with a half-life of 10, 9, 8, and 4 h, respectively. Influence of metal ions on enzyme activity revealed that Ca+2 enhances greatly the relative activity to 151.3 %; whereas Hg2+ inhibited significantly the enzyme. At the best of our knowledge, this is the first report on the production of xylanase by the thermophilic bacterium C. algeriensis. This thermo- and alkaline-tolerant xylanase could be used in pulp bleaching process.

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Acknowledgments

We wish to express our gratitude to Abdelhak Kouchah (Hyproc Shiping Company) for his valuable help during the preparation of this work.

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Authors and Affiliations

  1. Laboratory of Cellular and Molecular Biology (Microbiology group), Faculty of Biology, University of Science and Technology Houari Boumediene, Bab Ezzouar, Algiers, Algeria

    Khelifa Bouacem, Amel Bouanane-Darenfed & Hocine Hacène

  2. Laboratory of Applied Microbiology, Faculty of Nature Science and Life, University A/Mira of Bejaia, Targa Ouzemmour, 06000, Algeria

    Nawel Boucherba, Mouloud Kecha & Said Benallaoua

  3. Aix Marseille Université, CNRS, Université de Toulon, IRD, Mediterranean Institute of Oceanography (MIO), UM 110, 13288, Marseille, France

    Amel Bouanane-Darenfed, Manon Joseph, Wajdi Ben Hania, Bernard Ollivier & Marie-Laure Fardeau

  4. Maquav, Bioqual Laboratory, INATAA, Université de Constantine 1, Route de Ain El-Bey, 25000, Constantine, Algerie

    Mohammed Gagaoua

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  1. Khelifa Bouacem
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Correspondence to Marie-Laure Fardeau.

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Bouacem, K., Bouanane-Darenfed, A., Boucherba, N. et al. Partial Characterization of Xylanase Produced by Caldicoprobacter algeriensis, a New Thermophilic Anaerobic Bacterium Isolated from an Algerian Hot Spring. Appl Biochem Biotechnol 174, 1969–1981 (2014). https://doi.org/10.1007/s12010-014-1153-2

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