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Caldicoprobacteralgeriensis sp. nov. a New Thermophilic Anaerobic, Xylanolytic Bacterium Isolated from an Algerian Hot Spring

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Abstract

A thermophilic anaerobic bacterium (strain TH7C1T) was isolated from the hydrothermal hot spring of Guelma in the northeast of Algeria. Strain TH7C1T stained Gram-positive, was a non-motile rod appearing singly, in pairs, or as long chains (0.7–1 × 2–6 μm2). Spores were never observed. It grew at temperatures between 55 and 75°C (optimum 65°C) and at pH between 6.2 and 8.3 (optimum 6.9). It did not require NaCl for growth, but tolerated it up to 5 g l−1. Strain TH7C1T is an obligatory heterotroph fermenting sugars including glucose, galactose, lactose, raffinose, fructose, ribose, xylose, arabinose, maltose, mannitol, cellobiose, mannose, melibiose, saccharose, but also xylan, and pyruvate. Fermentation of sugars only occurred in the presence of yeast extract (0.1%). The end-products from glucose fermentation were acetate, lactate, ethanol, CO2, and H2. Nitrate, nitrite, thiosulfate, elemental sulfur, sulfate, and sulfite were not used as electron acceptors. The G+C content of the genomic DNA was 44.7 mol% (HPLC techniques). Phylogenetic analysis of the small-subunit ribosomal RNA (rRNA) gene sequence indicated that strain TH7C1T was affiliated to Firmicutes, order Clostridiales, family Caldicoprobacteraceae, with Caldicoprobacter oshimai (98.5%) being its closest relative. Based on phenotypic, phylogenetic, and genetic characteristics, strain TH7C1T is proposed as a novel species of genus Caldicoprobacter, Caldicoprobacter algeriensis, sp. nov. (strain TH7C1T = DSM 22661T = JCM 16184T).

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Authors and Affiliations

  1. Laboratoire de Biologie Cellulaire et Moléculaire (équipe de Microbiologie), Université des sciences et de la technologie Houari Boumédiènne, Bab Ezzouar, Algiers, Algeria

    Amel Bouanane-Darenfed, Salima Kebbouche-Gana & Hocine Hacene

  2. Laboratoire de Microbiologie IRD-ESIL, UMR 180, Universités de Provence et de la Méditerranée, ESIL case 925, 163 avenue de Luminy, 13288, Marseille Cedex 9, France

    Amel Bouanane-Darenfed, Marie-Laure Fardeau, Patrick Grégoire, Manon Joseph, Jean-Luc Cayol & Bernard Ollivier

  3. Laboratoire de la Sécurité Alimentaire et de l’Environnement-Police Scientifique, Algiers, Algeria

    Tahar Benayad

Authors
  1. Amel Bouanane-Darenfed
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  2. Marie-Laure Fardeau
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  9. Bernard Ollivier
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Correspondence to Bernard Ollivier.

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Bouanane-Darenfed, A., Fardeau, ML., Grégoire, P. et al. Caldicoprobacteralgeriensis sp. nov. a New Thermophilic Anaerobic, Xylanolytic Bacterium Isolated from an Algerian Hot Spring. Curr Microbiol 62, 826–832 (2011). https://doi.org/10.1007/s00284-010-9789-9

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