Abstract
Consolidated bioprocessing of lignocellulose for ethanol production is realized by expressing cellulase enzymes on ethanologenic strain. In this study, an ethanologenic Escherichia coli ZY81 was constructed by integrating pyruvate decarboxylase gene pdc and alcohol dehydrogenase gene adhB from Zymomonas mobilis into the genome of E. coli JM109 to obtain the capability of ethanol production. Then, the β-glucosidase gene bglB from Bacillus polymyxa was cloned and secretively expressed in E. coli ZY81. The recombinant strain E. coli ZY81/bglB showed an obvious activity of β-glucosidase in extracellular location with more than half in periplasmic space. EDTA was found to promote the release of the periplasmic proteins by approximately tenfold. E. coli ZY81/bglB utilized cellobiose as sole carbon source for ethanol production with 33.99 % of theoretical yield.
Similar content being viewed by others
References
Ryu, S. H., & Karim, M. N. (2011). Applied Microbiology and Biotechnology, 91, 529–542.
Wen, F., Nair, N. U., & Zhao, H. (2009). Current Opinion in Biotechnology, 20, 412–419.
Lynd, L. R. (1996). Annual Review of Energy and the. Environment, 21, 403–465.
Lynd, L. R., Weimer, P. J., & Zyl, W. H. (2002). Microbiology and Molecular Biology Reviews, 66, 506–577.
Grange, D., Haan, R., & Zyl, W. H. (2010). Applied Microbiology and Biotechnology, 87, 1195–1208.
Lynd, L. R., Zyl, W. H., McBride, J. E., & Laser, M. (2005). Current Opinion in Biotechnology, 16, 577–583.
Ragauskas, A. J., Williams, C. K., Davison, B. H., Britovsek, G., Cairney, J., Eckert, C. A., Hallett, J. P., Leak, D. J., Liotta, C. L., Mielenz, H. R., Murphy, R., Templer, R., & Tschaplinski, T. (2006). Science, 311, 484–489.
Sharrock, K. (1988). Journal of Biochemical and Biophysical Methods, 17, 81–105.
Xu, Q., Singh, A., & Himmel, M. E. (2009). Current Opinion in Biotechnology, 20, 364–371.
Zhang, X. Z., & Zhang, Y. H. (2010). Engineering in Life Sciences, 10, 398–406.
Shewale, J. G. (1982). International Journal of Biochemistry and Cell Biology, 14, 435–443.
Weber, C., Farwick, A., Benisch, F., Brat, D., Dietz, H., Subtil, T., & Boles, E. (2010). Applied Microbiology and Biotechnology, 87, 1303–1315.
Stephanopoulos, G. (2007). Science, 315, 801–804.
Yan, S. M., & Wu, G. (2013). Biotechnology for Biofuels., 6, 177.
Adin, D. M., Visick, K. L., & Stabb, E. V. (2008). Applied and Environmental Microbiology, 74, 4059–4069.
Parisutham, V., & Lee, S. K. (2011). Applied Microbiology and Biotechnology, 92, 125–132.
Liu, J. M., Xin, X. J., Li, C. X., Xu, J. H., & Bao, J. (2012). Applied Biochemistry and Biotechnology, 166, 652–662.
Kirill, A. D., & Barry, L. W. (2002). Proceedings of the National Academy of Sciences of the United States of America, 12, 6640–6645.
Shefali, G., Nidhi, A., & Syed, S. Y. (2013). Protein Expression and Purification, 88, 20–25.
Ohta, K., Alterthum, F., & Ingram, L. O. (1990). Applied and Environmental Microbiology, 56, 463–465.
Dien, B. S., Hespell, R. B., Wyckoff, H. A., & Bothast, R. J. (1998). Enzyme and Microbial Technology, 23, 366–371.
Causey, T. B., Shanmugam, K. T., Yomano, L. P., & Ingram, L. O. (2004). Proceedings of the National Academy of Sciences of the United States of America, 101, 2235–2240.
Martinez, A., Grabar, T. B., Shanmugam, K. T., Yomano, L. P., York, S. W., & Ingram, L. O. (2007). Biotechnology Letters, 29, 397–404.
Kim, Y., Ingram, L. O., & Shanmugam, K. T. (2007). Applied and Environmental Microbiology, 73, 1766–1771.
Dien, B. S., Nichols, N. N., O’Bryan, P. J., & Bothast, R. J. (2000). Applied Biochemistry and Biotechnology, 84–86, 181–196.
Orencio-Trejo, M., Flores, N., Escalante, A., Hernández-Chávez, G., Bolívar, F., Gosset, G., & Martinez, A. (2008). Biotechnology for Biofuels., 1, 8.
Kotarska, K., Czupryn´ski, B., & Kłosowski, G. (2006). Journal of Food Engineering, 77, 965–971.
Lindsay, S. E., Bothast, R. J., & Ingram, L. O. (1995). Applied Microbiology and Biotechnology, 43, 70–75.
Alterthum, F., & Ingram, L. O. (1989). Applied and Environmental Microbiology, 55, 1943–1948.
Underwood, S. A., Buszko, M. L., Shanmugam, K. T., & Ingram, L. O. (2004). Applied and Environmental Microbiology, 70, 2734–2740.
Underwood, S. A., Zhou, S., Causey, T. B., Yomano, L. P., Shanmugam, K. T., & Ingram, L. O. (2002). Applied and Environmental Microbiology, 68, 6263–6272.
Lawford, H. G., & Rousseau, J. D. (1991). Applied Biochemistry and Biotechnology, 28, 221–236.
Chambergo, F. S., Bonaccorsi, E. D., Ferreira, A. J. S., Ramos, A. S. P., Ferreira Júnior, J. R., Abrahão-Neto, J., Farah, J. P., & El-Dorry, H. (2002). Journal of Biological Chemistry, 277, 13983–13988.
Fuhrer, T., & Sauer, U. (2009). Journal of Bacteriology, 191, 2112–2121.
Mergulhao, F. J. M., Summersb, D. K., & Monteiro, G. A. (2005). Biotechnology Advances, 23, 177–202.
Choi, J. H., & Lee, S. Y. (2004). Applied Microbiology and Biotechnology, 64, 625–635.
Shokri, A., Sanden, A. M., & Larsson, G. (2003). Applied Microbiology and Biotechnology, 60, 654–664.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Luo, Z., Zhang, Y. & Bao, J. Extracellular Secretion of β-glucosidase in Ethanologenic E. coli Enhances Ethanol Fermentation of Cellobiose. Appl Biochem Biotechnol 174, 772–783 (2014). https://doi.org/10.1007/s12010-014-1108-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12010-014-1108-7