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Mutation Breeding of Lycopene-Producing Strain Blakeslea Trispora by a Novel Atmospheric and Room Temperature Plasma (ARTP)

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Abstract

To improve the fermentation efficiency of lycopene, a plasma jet, driven by an active helium atom supplied with atmospheric and room temperature plasma (ARTP) biological breeding system, was used as a new method to generate mutations in Blakeslea trispora (−). After several rounds of screening, a mutant A5 with high concentration of lycopene and dry biomass was isolated, which showed a maximum lycopene concentration (26.4 ± 0.2 mg/g dry biomass) which was 55 % higher than the parent strain (16.9 ± 0.3 mg/g dry biomass) in the production of lycopene. Compared with parent strain, B. trispora A5 required less dissolved oxygen (10 % less than that of parent strain) to reach maximum concentration in a 5-L stirred tank reactor batch fermentation.

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Acknowledgments

This work was supported by the Natural Science Foundation of Jiangsu Province, China (Grant No. BK20130130), the Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China (Grant No. KLIBKF201105), and the Project Funded by China Postdoctoral Science Foundation (Grant No. 2014M550264).

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Authors and Affiliations

  1. The Key Laboratory of Industrial Biotechnology, Ministry of Education, and School of Biotechnology, Jiangnan University, Wuxi, 214122, Jiangsu Province, China

    Wang Qiang, Feng Ling-ran, Wei Luo, Li Han-guang, Wang Lin, Zhou Ya & Yu Xiao-bin

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  1. Wang Qiang
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  2. Feng Ling-ran
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  3. Wei Luo
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  4. Li Han-guang
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  5. Wang Lin
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  6. Zhou Ya
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  7. Yu Xiao-bin
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Correspondence to Yu Xiao-bin.

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Qiang, W., Ling-ran, F., Luo, W. et al. Mutation Breeding of Lycopene-Producing Strain Blakeslea Trispora by a Novel Atmospheric and Room Temperature Plasma (ARTP). Appl Biochem Biotechnol 174, 452–460 (2014). https://doi.org/10.1007/s12010-014-0998-8

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  • DOI: https://doi.org/10.1007/s12010-014-0998-8

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