Abstract
With 30 g glucose/l as carbon source, Clostridium beijerinckii ART124, a mutant created by atmospheric pressure glow discharge, produced 13.7 g total solvent/l (containing 3.1 g acetone/l, 10.4 g butanol/l and 0.2 g ethanol/l) in 72 h. The mutant could also use sucrose or xylose or a mixture of glucose/xylose/arabinose with nearly equal yields.
Similar content being viewed by others
References
Annous BA, Blaschek HP (1991) Isolation and characterization of Clostridium acetobutylicum mutants with enhanced amylolytic activity. Appl Environ Microbiol 57:2544–2548
Chen CK, Blaschek HP (1999) Acetate enhances solvent production and prevents degeneration in Clostridium beijerinckii BA101. Appl Microbiol Biotechnol 52:170–173
Ezejia TC, Qureshib N, Blascheka HP (2005) Continuous butanol fermentation and feed starch retrogradation: butanol fermentation sustainability using Clostridium beijerinckii BA101. J Biotechnol 115:179–187
Formanek J, Mackie R, Blaschek HP (1997) Enhanced butanol production by Clostridium beijerinckii BA101 grown in semi-defined P2 medium containing 6 percent maltodextrin or glucose. Appl Environ Microbiol 63:2306–2310
Hua XF, Wang J, Wu ZJ, Zhang HX, Li HP, Xing XH, Liu Z (2010) A salt tolerant Enterobacter cloacae mutant for bioaugmentation of petroleum- and salt-contaminated soil. Biochem Eng J 49:201–206
Jin XQ, Zhou H, Wu X, Zhang G, He BF (2008) A rapid screening method of producing strain in acetone − butanol fermentation. Chin J Process Eng 6:1185–1189
Jones DT, Woods DR (1986) Acetone–butanol fermentation revisited. Microbiol Rev 50:484–524
Laroussi M (2005) Low temperature plasma-based sterilization: overview and state-of-the-art. Plasma Proc Polym 2:391–400
Li G, Li HP, Wang YL, Wang S, Zhao HX, Sun WT, Xing XH, Bao CY (2008) Genetic effects of radio-frequency, atmospheric-pressure glow discharges with helium. Appl Phys Lett 92:2551504
Maddox IS (1980) Production of n-butanol from whey filtrate using Clostridium acetobutylicum NCIB 2951. Biotechnol Lett 2:493–498
Mutschlechner O, Swoboda H, Gapes JR (2000) Continuous two-stage ABE-fermentation using Clostridium beijerinckii NRRL B592 operating with a growth rate in the first stage vessel close to its maximal value. J Mol Microbiol Biotechnol 2:101–105
Qureshi N, Blaschek HP (2001) Recent advances in ABE fermentation: hyper-butanol producing Clostridium beijerinckii BA101. J Ind Microbiol Biotechnol 27:287–291
Shao L, Hu S, Yang Y, Gu Y, Chen J, Yang YL, Jiang WH, Yang S (2007) Targeted gene disruption by use of a group II intron (targetron) vector in Clostridium acetobutylicum. Cell Res 17:963–965
Wang LY, Huang ZL, Li G, Zhao HX, Xing HX, Sun WT, Li HP, Gou ZX, Bao CY (2010) Novel mutation breeding method for Streptomyces avermitilis using an atmospheric pressure glow discharge plasma. J Appl Microbiol 108:851–858
Zhang YF, Chen J, Yang YL, Chiao JS (1996) The screening and application of the Clostridium acetobutylicum with high butanol ratio. Ind Microbiol 26:1–6
Acknowledgements
This work was supported by the “973” program of China (grant no. 2011CB70745), the State Key Laboratory of Materials-Oriented Chemical Engineering Foundation of Nanjing University of Technology, “Qinglan project” of Jiangsu province, “The six talent summit” of Jiangsu province (grant no. 06-A-047) and Commonweal research of CAF (201004001).
Author information
Authors and Affiliations
Corresponding author
Additional information
Ting Guo and Yan Tang contributed equally to this work.
Rights and permissions
About this article
Cite this article
Guo, T., Tang, Y., Xi, Yl. et al. Clostridium beijerinckii mutant obtained by atmospheric pressure glow discharge producing high proportions of butanol and solvent yields. Biotechnol Lett 33, 2379–2383 (2011). https://doi.org/10.1007/s10529-011-0702-9
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10529-011-0702-9