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Cloning of LicB from Clostridium thermocellum and its efficient secretive expression of thermostable β-1,3-1,4-glucanase

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Abstract

β-1,3-1,4-glucanase is a widely used enzyme in brewing and in animal feed processing. To produce the bacterial enzyme at an industrial scale, the enzyme should be able to be secreted from microbial cells into fermentation broth and be stable in different conditions. In this study, the LicB gene encoding β-1,3-1,4-glucanase (lichenase) from Clostridium thermocellum was secretively expressed in a secretive strain, Bacillus subtilis WB800, with eight extracellular protease deletion which made LicB expressed obviously and reached 1.18 U/g cell mass. The secreted β-1,3-1,4-glucanase was found to be active from 40 °C to 80 °C and achieved the optimal activity at 80 °C. The enzyme also has a wide pH range (pH 4–11). The most common metal ions and chemicals were found to be inert on its activity. The property of LicB-encoded β-1,3-1,4-glucanase and its efficient secretive expression makes it a potential enzyme for industrial production and application.

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Acknowledgments

This research was supported by the China Postdoctoral Science Foundation (2012M520850) and the Fundamental Research Funds for the Central Universities of China (WF0913005/1214025).

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai, 200237, China

    Zichen Luo, Qiuqiang Gao & Jie Bao

  2. Youtell Biotech Co, 526 Ruiqing Road, Pudong District, Shanghai, 201201, China

    Xinliang Li

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  1. Zichen Luo
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  2. Qiuqiang Gao
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  3. Xinliang Li
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  4. Jie Bao
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Correspondence to Jie Bao.

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Luo, Z., Gao, Q., Li, X. et al. Cloning of LicB from Clostridium thermocellum and its efficient secretive expression of thermostable β-1,3-1,4-glucanase. Appl Biochem Biotechnol 173, 562–570 (2014). https://doi.org/10.1007/s12010-014-0863-9

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  • DOI: https://doi.org/10.1007/s12010-014-0863-9

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