Skip to main content

Advertisement

SpringerLink
Log in

Rapid Isolation of a Facultative Anaerobic Electrochemically Active Bacterium Capable of Oxidizing Acetate for Electrogenesis and Azo Dyes Reduction

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

In this study, 27 strains of electrochemically active bacteria (EAB) were rapidly isolated and their capabilities of extracellular electron transfer were identified using a photometric method based on WO3 nanoclusters. These strains caused color change of WO3 from white to blue in a 24-well agar plate within 40 h. Most of the isolated EAB strains belonged to the genera of Aeromonas and Shewanella. One isolate, Pantoea agglomerans S5-44, was identified as an EAB that can utilize acetate as the carbon source to produce electricity and reduce azo dyes under anaerobic conditions. The results confirmed the capability of P. agglomerans S5-44 for extracellular electron transfer. The isolation of this acetate-utilizing, facultative EBA reveals the metabolic diversity of environmental bacteria. Such strains have great potential for environmental applications, especially at interfaces of aerobic and anaerobic environments, where acetate is the main available carbon source.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Bond, D. R., & Lovley, D. R. (2003). Applied and Environmental Microbiology, 69, 1548–1555.

    Article  CAS  Google Scholar 

  2. Bretschger, O., Obraztsova, A., Sturm, C. A., Chang, I. S., Gorby, Y. A., Reed, S. B., Culley, D. E., Reardon, C. L., Barua, S., Romine, M. F., Zhou, J., Beliaev, A. S., Bouhenni, R., Saffarini, D., Mansfeld, F., Kim, B. H., Fredrickson, J. K., & Nealson, K. H. (2007). Applied and Environmental Microbiology, 73, 7003–7012.

    Article  CAS  Google Scholar 

  3. Caccavo, F., Coates, J. D., RosselloMora, R. A., Ludwig, W., Schleifer, K. H., Lovley, D. R., & McInerney, M. J. (1996). Archives of Microbiology, 165, 370–376.

    Article  CAS  Google Scholar 

  4. Cai, P. J., Xiao, X., He, Y. R., Li, W. W., Chu, J., Wu, C., He, M. X., Zhang, Z., Sheng, G. P., Lam, M. H. W., Xu, F., & Yu, H. Q. (2012). Applied Microbiology and Biotechnology, 93, 1769–1776.

    Article  CAS  Google Scholar 

  5. Coates, J. D., Ellis, D. J., Gaw, C. V., & Lovley, D. R. (1999). International Journal of Systematic Bacteriology, 49, 1615–1622.

    Article  CAS  Google Scholar 

  6. Cummings, D. E., Caccavo, F., Spring, S., & Rosenzweig, R. F. (1999). Archives of Microbiology, 171, 183–188.

    Article  CAS  Google Scholar 

  7. Francis, C. A., Obraztsova, A. Y., & Tebo, B. M. (2000). Applied and Environmental Microbiology, 66, 543–548.

    Article  CAS  Google Scholar 

  8. Freguia, S., Teh, E. H., Boon, N., Leung, K. M., Keller, J., & Rabaey, K. (2010). Bioresource Technology, 101, 1233–1238.

    Article  CAS  Google Scholar 

  9. Holmes, D. E., Nicoll, J. S., Bond, D. R., & Lovley, D. R. (2004). Applied and Environmental Microbiology, 70, 6023–6030.

    Article  CAS  Google Scholar 

  10. Hong, Y. G., Guo, J., Xu, Z. C., Mo, C. Y., Xu, M. Y., & Sun, G. P. (2007). Applied Microbiology and Biotechnology, 75, 647–654.

    Article  CAS  Google Scholar 

  11. Jiang, D., Li, B., Jia, W., & Lei, Y. (2010). Applied Biochemistry and Biotechnology, 160, 182–196.

    Article  CAS  Google Scholar 

  12. Kashefi, K., Holmes, D. E., Baross, J. A., & Lovley, D. R. (2003). Applied and Environmental Microbiology, 69, 2985–2993.

    Article  CAS  Google Scholar 

  13. Lee, J. Y., Phung, N. T., Chang, I. S., Kim, B. H., & Sung, H. C. (2003). FEMS Microbiology Letters, 223, 185–191.

    Article  CAS  Google Scholar 

  14. Park, H. S., Kim, B. H., Kim, H. S., Kim, H. J., Kim, G. T., Kim, M., Chang, I. S., Park, Y. K., & Chang, H. I. (2001). Anaerobe, 7, 297–306.

    Article  CAS  Google Scholar 

  15. Pearce, C. I., Christie, R., Boothman, C., von Canstein, H., Guthrie, J. T., & Lloyd, J. R. (2006). Biotechnology and Bioengineering, 95, 692–703.

    Article  CAS  Google Scholar 

  16. Pham, C. A., Jung, S. J., Phung, N. T., Lee, J., Chang, I. S., Kim, B. H., Yi, H., & Chun, J. (2003). FEMS Microbiology Letters, 223, 129–134.

    Article  CAS  Google Scholar 

  17. Sarayu, K., & Sandhya, S. (2012). Applied Biochemistry and Biotechnology, 167, 645–661.

    Article  CAS  Google Scholar 

  18. Tebo, B. M., & Obraztsova, A. Y. (1998). FEMS Microbiology Letters, 162, 193–198.

    Article  CAS  Google Scholar 

  19. Tong, Z. H., Bischoff, M., Nies, L., Applegate, B., & Turco, R. F. (2007). Environmental Science and Technology, 41, 2985–2991.

    Article  CAS  Google Scholar 

  20. Xu, M. Y., Guo, J., Cen, Y. H., Zhong, X. Y., Cao, W., & Sun, G. P. (2005). International Journal of Systematic and Evolutionary Microbiology, 55, 363–368.

    Article  CAS  Google Scholar 

  21. Yuan, S. J., Li, W. W., Cheng, Y. Y., He, H., Chen, J. J., Tong, Z. H., Lin, Z. Q., Zhang, F., Sheng, G. P., & Yu, H. Q. (2014). Nature Protocols, 9, 112–119.

  22. Zhang, T., Cui, C., Chen, S., Yang, H., & Shen, P. (2008). Electrochemistry Communications, 10, 293–297.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

The authors wish to thank the National Natural Science Foundation of China (21007064) and the Fundamental Research Funds for the Central Universities (WK2060190010) for the partial support of this study.

Author information

Authors and Affiliations

  1. Department of Chemistry, University of Science & Technology of China, Hefei, 230026, China

    Nan Shen, Shi-Jie Yuan, Chao Wu, Yuan-Yuan Cheng, Xiang-Ning Song, Wen-Wei Li, Zhong-Hua Tong & Han-Qing Yu

Authors
  1. Nan Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Shi-Jie Yuan
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Chao Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yuan-Yuan Cheng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiang-Ning Song
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Wen-Wei Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Zhong-Hua Tong
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Han-Qing Yu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yuan-Yuan Cheng.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shen, N., Yuan, SJ., Wu, C. et al. Rapid Isolation of a Facultative Anaerobic Electrochemically Active Bacterium Capable of Oxidizing Acetate for Electrogenesis and Azo Dyes Reduction. Appl Biochem Biotechnol 173, 461–471 (2014). https://doi.org/10.1007/s12010-014-0853-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-014-0853-y

Keywords

Search

Navigation