Skip to main content
SpringerLink
Log in

Exoelectrogenic Bacterium Phylogenetically Related to Citrobacter freundii, Isolated from Anodic Biofilm of a Microbial Fuel Cell

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

An electrogenic bacterium, named Citrobacter freundii Z7, was isolated from the anodic biofilm of microbial fuel cell (MFC) inoculated with aerobic sewage sludge. Cyclic voltammetry (CV) analysis exhibited that the strain Z7 had relatively high electrochemical activity. When the strain Z7 was inoculated into MFC, the maximum power density can reach 204.5 mW/m2 using citrate as electron donor. Series of substrates including glucose, glycerol, lactose, sucrose, and rhammose could be utilized to generate power. CV tests and the addition of anode solution as well as AQDS experiments indicated that the strain Z7 might transfer electrons indirectly via secreted mediators.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Similar content being viewed by others

References

  1. Logan, B. E. (2009). Nature Reviews Microbiology, 7, 375–381.

    Article  CAS  Google Scholar 

  2. Bond, D. R., Holmes, D. E., Tender, L. M., & Lovley, D. R. (2002). Science, 295, 483–485.

    Article  CAS  Google Scholar 

  3. Holmes, D. E., Bond, D. R., & Lovley, D. R. (2004). Applied and Environmental Microbiology, 70, 1234–1237.

    Article  CAS  Google Scholar 

  4. Kim, B. H., Kim, H. J., Hyun, M. S., & Park, D. H. (1999). Journal of Microbiology and Biotechnology, 9, 127–131.

    Google Scholar 

  5. Wang, A., Liu, L., Sun, D., Ren, N., & Lee, D. J. (2010). International Journal of Hydrogen Energy, 35, 3178–3182.

    Article  CAS  Google Scholar 

  6. Xu, S., & Liu, H. (2011). Journal of Applied Microbiology, 111, 1108–1115.

    Article  CAS  Google Scholar 

  7. Zuo, Y., Xing, D., Regan, J. M., & Logan, B. E. (2008). Applied and Environmental Microbiology, 74, 3130–3137.

    Article  CAS  Google Scholar 

  8. Rabaey, K., Boon, N., Siciliano, S. D., Verhaege, M., & Verstraete, W. (2004). Applied and Environmental Microbiology, 70, 5373–5382.

    Article  CAS  Google Scholar 

  9. Zhang, T., Cui, C., Chen, S., Yang, H., & Shen, P. (2008). Electrochemistry Communications, 10, 293–297.

    Article  CAS  Google Scholar 

  10. Rezaei, F., Xing, D., Wagner, R., Regan, J. M., Richard, T. L., & Logan, B. E. (2009). Applied and Environmental Microbiology, 75, 3673–3678.

    Article  CAS  Google Scholar 

  11. Xia, X., Cao, X. X., Liang, P., Huang, X., Yang, S. P., & Zhao, G. G. (2010). Applied Microbiology and Biotechnology, 87, 383–390.

    Article  CAS  Google Scholar 

  12. Zhang, L., Zhou, S., Zhuang, L., Li, W., Zhang, J., Lu, N., & Deng, L. (2008). Electrochemistry Communications, 10, 1641–1643.

    Article  CAS  Google Scholar 

  13. Xing, D., Cheng, S., Logan, B. E., & Regan, J. M. (2010). Applied Microbiology and Biotechnology, 85, 1575–1587.

    Article  CAS  Google Scholar 

  14. Pham, C. A., Jung, S. J., Phung, N. T., Lee, J., Chang, I. S., Kim, B. H., Yi, H., & Chun, J. (2003). FEMS Microbiology Letters, 223, 129–134.

    Article  CAS  Google Scholar 

  15. Holmes, D. E., Nicoll, J. S., Bond, D. R., & Lovley, D. R. (2004). Applied and Environmental Microbiology, 70, 6023–6030.

    Article  CAS  Google Scholar 

  16. Chaudhuri, S. K., & Lovley, D. R. (2003). Nature Biotechnology, 21, 1229–1232.

    Article  CAS  Google Scholar 

  17. Xing, D., Zuo, Y., Cheng, S., Regan, J. M., & Logan, B. E. (2008). Environmental Science and Technology, 42, 4146–4151.

    Article  CAS  Google Scholar 

  18. Borole, A. P., O’Neill, H., Tsouris, C., & Cesar, S. (2008). Biotechnology Letters, 30, 1367–1372.

    Article  CAS  Google Scholar 

  19. Fedorovich, V., Knighton, M. C., Pagaling, E., Ward, F. B., Free, A., & Goryanin, I. (2009). Applied and Environmental Microbiology, 75, 7326–7334.

    Article  CAS  Google Scholar 

  20. Coates, J. D., Ellis, D. J., Gaw, C. V., & Lovley, D. R. (1999). International Journal of Systematic Bacteriology, 49, 1615–1622.

    Article  CAS  Google Scholar 

  21. Park, H. S., Kim, B. H., Kim, H. S., Kim, H. J., Kim, G. T., Kim, M., Chang, I. S., Park, Y. K., & Chang, H. I. (2001). Anaerobe, 7, 297–306.

    Article  CAS  Google Scholar 

  22. Wrighton, K. C., Agbo, P., Warnecke, F., Weber, K. A., Brodie, E. L., DeSantis, T. Z., Hugenholtz, P., Andersen, G. L., & Coates, J. D. (2008). ISME Journal, 2, 1146–1156.

    Article  CAS  Google Scholar 

  23. Nimje, V. R., Chen, C. Y., Chen, C. C., Jean, J. S., Reddy, A. S., Fan, C. W., Pan, K. Y., Liu, H. T., & Chen, J. L. (2009). Journal of Power Sources, 190, 258–263.

    Article  CAS  Google Scholar 

  24. Bond, D. R., & Lovley, D. R. (2003). Applied and Environmental Microbiology, 69, 1548–1555.

    Article  CAS  Google Scholar 

  25. Malvankar, N. S., Vargas, M., Nevin, K. P., Franks, A. E., Leang, C., Kim, B. C., Inoue, K., Mester, T., Covalla, S. F., Johnson, J. P., Rotello, V. M., Tuominen, M. T., & Lovley, D. R. (2011). Nature Nanotechnology, 6, 573–579.

    Article  Google Scholar 

  26. Reguera, G., McCarthy, K. D., Mehta, T., Nicoll, J. S., Tuominen, M. T., & Lovley, D. R. (2005). Nature, 435, 1098–1101.

    Article  CAS  Google Scholar 

  27. Marsili, E., Baron, D. B., Shikhare, I. D., Coursolle, D., Gralnick, J. A., & Bond, D. R. (2008). Proceedings of the National Academy of Sciences USA, 105, 3968–3973.

    Article  CAS  Google Scholar 

  28. Gorby, Y. A., Yanina, S., McLean, J. S., Rosso, K. M., Moyles, D., Dohnalkova, A., Beveridge, T. J., Chang, I. S., Kim, B. H., Kim, K. S., Culley, D. E., Reed, S. B., Romine, M. F., Saffarini, D. A., Hill, E. A., Shi, L., Elias, D. A., Kennedy, D. W., Pinchuk, G., Watanabe, K., Ishii, S. I., Logan, B., Nealson, K. H., & Fredrickson, J. K. (2006). Proceedings of the National Academy of Sciences, 103, 11358–11363.

    Article  CAS  Google Scholar 

  29. Brenner, D. J., Grimont, P. A., Steigerwalt, A. G., Fanning, G. R., Ageron, E., & Riddle, C. F. (1993). International Journal of Systematic Bacteriology, 43, 645–658.

    Article  CAS  Google Scholar 

  30. Zhu, N., Chen, X., Zhang, T., Wu, P., Li, P., & Wu, J. (2011). Bioresource Technology, 102, 422–426.

    Article  CAS  Google Scholar 

  31. Min, B., & Logan, B. E. (2004). Environmental Science and Technology, 38, 5809–5814.

    Article  CAS  Google Scholar 

  32. Savelieva, O., Kotova, I., Roelofsen, W., Stams, A. J. M., & Netrusov, A. (2004). Archives of Microbiology, 181, 163–170.

    Article  CAS  Google Scholar 

  33. Wang, J. T., Chang, S. C., Chen, Y. C., & Luh, K. T. (2000). Journal of Microbiology, Immunology and Infection, 33, 258–262.

    CAS  Google Scholar 

  34. Chae, K. J., Choi, M. J., Lee, J. W., Kim, K. Y., & Kim, I. S. (2009). Bioresource Technology, 100, 3518–3525.

    Article  CAS  Google Scholar 

  35. Puchenkova, S. G. (1996). Mikrobiolohichnyĭ Zhurnal, 58, 3–7.

    CAS  Google Scholar 

  36. Zhang, J., Zhang, E., Scott, K., & Burgess, J. G. (2012). Environmental Science and Technology, 46, 2984–2992.

    Article  CAS  Google Scholar 

  37. Morris, J. M., Jin, S., Crimi, B., & Pruden, A. (2009). Chemical Engineering Journal, 146, 161–167.

    Article  CAS  Google Scholar 

  38. Reguera, G., Pollina, R. B., Nicoll, J. S., & Lovley, D. R. (2007). Journal of Bacteriology, 189, 2125–2127.

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This study was financially supported by National Natural Science Foundation of China (30800796 and 31272482), the Fundamental Research Funds for the Central Universities (2014ZG015), and the Program for New Century Excellent Talents in University (NCET-11-0166).

Author information

Authors and Affiliations

  1. School of Environment and Energy, South China University of Technology, Guangzhou, 510006, People’s Republic of China

    Jianjian Huang, Nengwu Zhu, Yanlan Cao, Yue Peng, Pingxiao Wu & Wenhao Dong

  2. The Key Laboratory of Pollution Control and Ecosystem Restoration in Industry Clusters of Ministry of Education, Guangzhou, 510006, People’s Republic of China

    Nengwu Zhu & Pingxiao Wu

Authors
  1. Jianjian Huang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nengwu Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yanlan Cao
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Yue Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Pingxiao Wu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Wenhao Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Nengwu Zhu.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Huang, J., Zhu, N., Cao, Y. et al. Exoelectrogenic Bacterium Phylogenetically Related to Citrobacter freundii, Isolated from Anodic Biofilm of a Microbial Fuel Cell. Appl Biochem Biotechnol 175, 1879–1891 (2015). https://doi.org/10.1007/s12010-014-1418-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-014-1418-9

Keywords

Search

Navigation