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Impact of Pretreatment with Dilute Sulfuric Acid Under Moderate Temperature on Hydrolysis of Corn Stover with Two Enzyme Systems

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Abstract

Pretreatment of corn stover with dilute sulfuric acid at moderate temperature was investigated, and glucan digestibility by Cellic CTec2 and Celluclast on the pretreated biomass was compared. Pretreatments were carried out from 60 to 180 min at the temperature from 105 to 135 °C, with acid concentrations ranging from 0.5 to 2 % (w/v). Significant portion of xylan was removed during pretreatment, and the glucan digestibility by CTec2 was significantly better than that by Celluclast in all cases. Analysis showed that glucan digestibility by both two enzymes correlated directly with the extent of xylan removal in pretreatment. Confidence interval was built to give a more precise range of glucan conversion and to test the significant difference among pretreatment conditions. Response surface model was built to obtain the optimal pretreatment condition to achieve high glucan conversion after enzymatic hydrolysis. Considering the cost and energy savings, the optimal pretreatment condition of 1.75 % acid for 160 min at 135 °C was determined, and glucan conversion can achieve the range from 72.86 to 76.69 % at 95 % confidence level after enzymatic hydrolysis, making total glucan recovery up to the range from 89.42 to 93.25 %.

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Acknowledgments

This work was carried out with funding from a strategic research grant from the Institute of Agriculture and Natural Resources at the University of Nebraska. The authors would like to thank Novozymes North America, Inc., for the generous offer of CTec2 and Mr. Yixiang Zhang for his assistance with statistical analysis.

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  1. Department of Biological Systems Engineering, University of Nebraska-Lincoln, Lincoln, NE, 68583, USA

    Chao Tai & Deepak Keshwani

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  1. Chao Tai
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  2. Deepak Keshwani
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Correspondence to Deepak Keshwani.

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Tai, C., Keshwani, D. Impact of Pretreatment with Dilute Sulfuric Acid Under Moderate Temperature on Hydrolysis of Corn Stover with Two Enzyme Systems. Appl Biochem Biotechnol 172, 2628–2639 (2014). https://doi.org/10.1007/s12010-013-0721-1

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  • DOI: https://doi.org/10.1007/s12010-013-0721-1

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