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Transesterification of Waste Cooking Oil by an Organic Solvent-Tolerant Alkaline Lipase from Streptomyces sp. CS273

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Abstract

The aim of this present study was to produce a microbial enzyme that can potentially be utilized for the enzymatic transesterification of waste cooking oil. To that end, an extracellular lipase was isolated and purified from the culture broth of Streptomyces sp. CS273. The molecular mass of purified lipase was estimated to be 36.55 kDa by SDS PAGE. The optimum lipolytic activity was obtained at alkaline pH 8.0 to 8.5 and temperature 40 °C, while the enzyme was stable in the pH range 7.0 ∼ 9.0 and at temperature ≤40 °C. The lipase showed highest hydrolytic activity towards p-nitrophenyl myristate (C14). The lipase activity was enhanced by several salts and detergents including NaCl, MnSo4, and deoxy cholic acid, while phenylmethylsulfonyl fluoride at concentration 10 mM inhibited the activity. The lipase showed tolerance towards different organic solvents including ethanol and methanol which are commonly used in transesterification reactions to displace alcohol from triglycerides (ester) contained in renewable resources to yield fatty acid alkyl esters known as biodiesel. Applicability of the lipase in transesterification of waste cooking oil was confirmed by gas chromatography mass spectrometry analysis.

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Acknowledgments

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MEST) (2010-0029178) and by research fund from Chosun University, 2013.

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Authors and Affiliations

  1. Department of Pharmacy, Chosun University, Gwangju, 501-759, Korea

    Poonam Mander, Yun Hee Choi & Jin Cheol Yoo

  2. Department of Chemical and Biological Engineering, Korea University, Seoul, Korea

    Hah-Young Yoo & Seung Wook Kim

  3. Department of Pharmacy, College of Pharmacy, Mokpo National University, Muan, Jeonnam, 534-729, Korea

    Seung Sik Cho

Authors
  1. Poonam Mander
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  2. Hah-Young Yoo
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  3. Seung Wook Kim
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  4. Yun Hee Choi
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  5. Seung Sik Cho
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  6. Jin Cheol Yoo
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Corresponding author

Correspondence to Jin Cheol Yoo.

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Poonam Mander and Hah-Young Yoo contributed equally to this work.

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Mander, P., Yoo, HY., Kim, S.W. et al. Transesterification of Waste Cooking Oil by an Organic Solvent-Tolerant Alkaline Lipase from Streptomyces sp. CS273. Appl Biochem Biotechnol 172, 1377–1389 (2014). https://doi.org/10.1007/s12010-013-0610-7

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  • DOI: https://doi.org/10.1007/s12010-013-0610-7

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