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An organic solvent-tolerant alkaline lipase from Streptomyces sp. CS268 and its application in biodiesel production

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Abstract

In an effort to identify a microbial lipase that can catalyze transesterification reactions used in biodiesel production, an organic solvent-tolerant lipase was purified from Streptomyces sp. CS268. The molecular weight of the purified lipase was estimated to be 37.5 kDa by SDS-PAGE. The lipase showed highest activity at a temperature of 30°C and pH 8.0 while it was stable in the pH range 4.0 ∼ 9.0 and at temperatures ≤ 50°C. It showed the highest hydrolytic activity towards medium-length acyl chain p-nitrophenyl decanoate with K m and V max values of 0.59 mM and 319.5 mmol/mg/min, respectively. Also, the lipase showed non-position specificity for triolein hydrolysis. The purified lipase catalyzed transesterification reaction of soybean oil with methanol, suggesting that it can be a potential enzymatic catalyst for biodiesel production.

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Authors and Affiliations

  1. Department of Pharmacy, College of Pharmacy, Chosun University, Gwangju, 501-759, Korea

    Poonam Mander, Seung Sik Cho, Jaya Ram Simkhada, Yun Hee Choi, Da Jeong Park, Jung Wan Ha & Jin Cheol Yoo

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  1. Poonam Mander
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  2. Seung Sik Cho
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  3. Jaya Ram Simkhada
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  4. Yun Hee Choi
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  5. Da Jeong Park
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  6. Jung Wan Ha
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  7. Jin Cheol Yoo
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Correspondence to Jin Cheol Yoo.

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Both authors contributed equally to this work.

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Mander, P., Cho, S.S., Simkhada, J.R. et al. An organic solvent-tolerant alkaline lipase from Streptomyces sp. CS268 and its application in biodiesel production. Biotechnol Bioproc E 17, 67–75 (2012). https://doi.org/10.1007/s12257-011-0347-5

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  • DOI: https://doi.org/10.1007/s12257-011-0347-5

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