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Purification and characterization of extracellular lipases from Pseudomonas monteilii TKU009 by the use of soybeans as the substrate

  • Original Paper
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Journal of Industrial Microbiology & Biotechnology

Abstract

A lipase-producing bacterium was isolated and identified as Pseudomonas monteilii TKU009. A lipase (F2) and lipase-like materials (F1) were purified from the culture supernatant of P. monteilii TKU009 with soybean powder as the sole carbon/nitrogen source. The molecular mass of F1 and F2 was estimated to be 44 kDa by SDS-PAGE and gel filtration. The optimum pH, optimum temperature, and pH and thermal stabilities of F2 were 7, 40°C, 8–11, and 50°C; and of F1 were 6, 40°C, 6–7, and 50°C, respectively. F2 was completely inhibited by EDTA and slightly by Mg2+, Fe2+, Mn2+, and SDS. F1 was completely inhibited by EDTA and Fe2+ and strongly by Zn2+, Mn2+, Ca2+, Mg2+, and SDS. The activities of both the enzymes were enhanced by the addition of non-ionic surfactants Triton X–100 and Tween 40, especially for F1. F2 preferably acted on substrates with a long chain (C10–C18) of fatty acids, while F1 showed a broad spectrum on those with chain length of C4–C18. The marked activity of F2 in organic solvents makes it an ideal choice for application in a water-restricted medium including organic synthesis.

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Acknowledgment

This work was supported in part by a grant from the National Science Council, Taiwan (NSC94-2313-B-212-003).

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Author notes

  1. San-Lang Wang

    Present address: , 151 Ying-Chuan Road, Tamsui, 251, Taiwan

Authors and Affiliations

  1. Graduate Institute of Life Sciences, Tamkang University, Tamsui, 251, Taiwan

    San-Lang Wang, Yu-Ting Lin, Tzu-Wen Liang, Sau-Hua Chio & Pei-Chen Wu

  2. Department of Chemistry, University of South Florida, Tampa, FL, 33620, USA

    Li-June Ming

  3. Department of Chemistry, National Cheng Kung University, Tainan, 701, Taiwan

    Li-June Ming

Authors
  1. San-Lang Wang
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  2. Yu-Ting Lin
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  3. Tzu-Wen Liang
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Correspondence to San-Lang Wang.

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Li-June Ming is a visiting Professor at the National Cheng Kung University.

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Wang, SL., Lin, YT., Liang, TW. et al. Purification and characterization of extracellular lipases from Pseudomonas monteilii TKU009 by the use of soybeans as the substrate. J Ind Microbiol Biotechnol 36, 65–73 (2009). https://doi.org/10.1007/s10295-008-0473-z

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  • DOI: https://doi.org/10.1007/s10295-008-0473-z

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