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Enhanced Resveratrol Production in Vitis vinifera Cell Suspension Cultures by Heavy Metals Without Loss of Cell Viability

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Abstract

The effects of heavy metal ions (Co2+, Ag+, Cd2+) on cell viability and secondary metabolite production, particularly anthocyanins and phenolic acids in Vitis vinifera cell suspension cultures, were investigated. Of these, Co at all three used concentrations (5.0, 25, and 50 μM), Ag, and Cd at low concentration (5.0 μM) were most effective to stimulate the phenolic acid production, increasing the 3-O-glucosyl-resveratrol up to 1.6-fold of the control level (250.5 versus 152.4 μmol/g), 4 h after the treatments. Meanwhile, the elicitors at effective concentrations did not suppress cell growth, while the cell viability maintained. In contrast, Ag and Cd at high concentrations (25 and 50 μM) remarkably reduced the cell viability, decreasing the cell viability up to about 15 % of the control level, 24 h after the treatments. The heavy metal ions did not affect the anthocyanin production. These observations show how, in a single system, different groups of secondary products can show distinct differences in their responses to potential elicitors. The 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity, peroxidase activity, medium pH value, and conductivity were only slightly elevated by the heavy metal ions. The results suggest that some of the secondary metabolites production was stimulated by the used elicitors, but there was not a stress response of the cells.

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Acknowledgments

We wish to thank Prof. Dietrich Knorr from Technical University Berlin for supporting this work. We also thank Irene Hemmerich from Technical University Berlin for providing V. vinifera cell culture and the continuous cultivation.

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Authors and Affiliations

  1. Institute of Food Biotechnology and Food Chemistry, Technical University Berlin, Königin-Luise Str. 22, 14195, Berlin, Germany

    Zhenzhen Cai, Anja Kastell & Iryna Smetanska

  2. National Graduate School of Engineering Chemistry of Lille, Avenue Dimitri Mendeleïev - Bâtiment C7 Cité Scientifique, BP 90108, 59652, Villeneuve d’Ascq cedex, France

    Claire Speiser

  3. Department of Plant Food Processing, University of Applied Science Weihenstephan-Triesdorf, Steingruber Str. 2, 91746, Weidenbach, Germany

    Iryna Smetanska

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  1. Zhenzhen Cai
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Correspondence to Zhenzhen Cai.

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Cai, Z., Kastell, A., Speiser, C. et al. Enhanced Resveratrol Production in Vitis vinifera Cell Suspension Cultures by Heavy Metals Without Loss of Cell Viability. Appl Biochem Biotechnol 171, 330–340 (2013). https://doi.org/10.1007/s12010-013-0354-4

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  • DOI: https://doi.org/10.1007/s12010-013-0354-4

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