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Optimization of a Natural Medium for Cellulase by a Marine Aspergillus niger Using Response Surface Methodology

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Abstract

The components of a natural medium were optimized to produce cellulase from a marine Aspergillus niger under solid state fermentation conditions by response surface methodology. Eichhornia crassipes and natural seawater were used as a major substrate and a source of mineral salts, respectively. Mineral salts of natural seawater could increase cellulase production. Raw corn cob and raw rice straw showed a significant positive effect on cellulase production. The optimum natural medium consisted of 76.9 % E. crassipes (w/w), 8.9 % raw corn cob (w/w), 3.5 % raw rice straw (w/w), 10.7 % raw wheat bran (w/w), and natural seawater (2.33 times the weight of the dry substrates). Incubation for 96 h in the natural medium increased the biomass to the maximum. The cellulase production was 17.80 U/g the dry weight of substrates after incubation for 144 h. The natural medium avoided supplying chemicals and pretreating substrates. It is promising for future practical fermentation of environment-friendly producing cellulase.

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Authors and Affiliations

  1. Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou, 310027, People’s Republic of China

    Dong-Sheng Xue, Hui-Yin Chen, Dong-Qiang Lin, Yi-Xin Guan & Shan-Jing Yao

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  1. Dong-Sheng Xue
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Correspondence to Shan-Jing Yao.

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Xue, DS., Chen, HY., Lin, DQ. et al. Optimization of a Natural Medium for Cellulase by a Marine Aspergillus niger Using Response Surface Methodology. Appl Biochem Biotechnol 167, 1963–1972 (2012). https://doi.org/10.1007/s12010-012-9734-4

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  • DOI: https://doi.org/10.1007/s12010-012-9734-4

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