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Improved Cellulase Production by Trichoderma reesei RUT C30 under SSF Through Process Optimization

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Abstract

The major constraint in the enzymatic saccharification of biomass for ethanol production is the cost of cellulase enzymes. Production cost of cellulases may be brought down by multifaceted approaches which includes the use of cheap lignocellulosic substrates for fermentation production of the enzyme, and the use of cost efficient fermentation strategies like solid state fermentation (SSF). The current study investigated the production of cellulase by Trichoderma reesei RUT C30 on wheat bran under SSF. Process parameters important in cellulase production were identified by a Plackett and Burman design and the parameters with significant effects on enzyme production were optimized for maximal yield using a central composite rotary design (CCD). Higher initial moisture content of the medium had a negative effect on production whereas incubation temperature influenced cellulase production positively in the tested range. Optimization of the levels of incubation temperature and initial moisture content of the medium resulted in a 6.2 fold increase in production from 0.605 to 3.8 U/gds of cellulase. The optimal combination of moisture and temperature was found to be 37.56% and 30 °C, respectively, for maximal cellulase production by the fungus on wheat bran.

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Authors and Affiliations

  1. Biotechnology Division, National Institute for Interdisciplinary Science and Technology (Formerly Regional Research Laboratory), Industrial Estate PO, Trivandrum, 695 019, India

    Reeta Rani Singhania, Rajeev K. Sukumaran & Ashok Pandey

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  1. Reeta Rani Singhania
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  2. Rajeev K. Sukumaran
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  3. Ashok Pandey
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Correspondence to Ashok Pandey.

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Singhania, R.R., Sukumaran, R.K. & Pandey, A. Improved Cellulase Production by Trichoderma reesei RUT C30 under SSF Through Process Optimization. Appl Biochem Biotechnol 142, 60–70 (2007). https://doi.org/10.1007/s12010-007-0019-2

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  • DOI: https://doi.org/10.1007/s12010-007-0019-2

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