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Improved Ethanol and Reduced Xylitol Production from Glucose and Xylose Mixtures by the Mutant Strain of Candida shehatae ATCC 22984

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Abstract

A mutant Cs3512, which showed better fermentation of xylose and the mixtures of xylose and glucose, was obtained through mutation of Candida shehatae ATCC 22984 and screening with a medium containing antimycin A and TTC (2,3,5-triphenyltetrazolium chloride). Cs3512 produced 44.4 g/l of ethanol from 121.3 g/l of xylose, which was 13% higher than that by ATCC 22984. At the same time, xylitol production was reduced by 38% to 10.2 g/l from 16.3 g/l by ATCC 22984. Cs3512 also showed 8% increase in ethanol yield from 0.39 to 0.42 g/g comparing to ATCC 22984 when fermenting the sugar mixture composed of 52.9 g/l glucose and 21.2 g/l xylose. When Cs3512 was used in the simultaneous saccharification and fermentation of lime pretreated rice straw via CaCCO (calcium capturing by carbonation) process, it produced ethanol at 77% of the theoretical yield. The results imply that Cs3512 is a potential non-recombinant yeast strain for ethanol production from lignocellulosic biomass.

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Acknowledgement

This work was supported by a grant from the Ministry of Agriculture, Forestry, and Fisheries of Japan (Rural Biomass Research Project, BEC-BA220).

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Authors and Affiliations

  1. National Food Research Institute, National Agriculture and Food Research Organization, 2-1-12 Kannondai, Tsukuba, Ibaraki, 305-8642, Japan

    Yuan Li, Jeung-yil Park, Riki Shiroma, Masakazu Ike & Ken Tokuyasu

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  1. Yuan Li
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  2. Jeung-yil Park
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  3. Riki Shiroma
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  4. Masakazu Ike
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  5. Ken Tokuyasu
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Correspondence to Ken Tokuyasu.

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Li, Y., Park, Jy., Shiroma, R. et al. Improved Ethanol and Reduced Xylitol Production from Glucose and Xylose Mixtures by the Mutant Strain of Candida shehatae ATCC 22984. Appl Biochem Biotechnol 166, 1781–1790 (2012). https://doi.org/10.1007/s12010-012-9586-y

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