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Efficient simultaneous saccharification and fermentation of agricultural residues bySaccharomyces cerevisiae andCandida shehatae

TheD-xylose fermenting yeast

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Abstract

Simultaneous Saccharification and Fermentation (SSF) experiments were carried out on agricultural residues using culture filtrate ofSclerotium rolfsii, which produces high levels of cellulases and hemicellulases for the saccharification of rice straw and bagasse, andCandida shehatae—the D-xylose fermenting yeast, andSaccharomyces cerevisiae, both separately and in coculture, for fermenting the released sugars. The coculture system showed efficient utilization of hydrolyzed sugars with 30–38% and 10–13% increase in ethanol production as compared toC. shehatae andS. cerevisiae, respectively, when cultivated separately. SSF simulation studies were carried out using standard sugar mixtures of glucose, xylose, and cellobiose. Both organisms could not use cellobiose, whereas glucose was used preferentially.C. shehatae was capable of utilizing xylose in the presence of glucose.

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Palnitkar, S.S., Lachke, A.H. Efficient simultaneous saccharification and fermentation of agricultural residues bySaccharomyces cerevisiae andCandida shehatae . Appl Biochem Biotechnol 26, 151–158 (1990). https://doi.org/10.1007/BF02921531

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