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Synthesis of Fructooligosaccharides from Aspergillus niger Commercial Inulinase Immobilized in Montmorillonite Pretreated in Pressurized Propane and LPG

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Abstract

Commercial inulinase from Aspergillus niger was immobilized in montmorillonite and then treated in pressurized propane and liquefied petroleum gas (LPG). Firstly, the effects of system pressure, exposure time, and depressurization rate, using propane and LPG, on enzymatic activity were evaluated through central composite design 23. Residual activities of 145.1 and 148.5 % were observed for LPG (30 bar, 6 h, and depressurization rate of 20 bar min−1) and propane (270 bar, 1 h, and depressurization rate of 100 bar min−1), respectively. The catalysts treated at these conditions in both fluids were then used for the production of fructooligosaccharides (FOS) using sucrose and inulin as substrates in aqueous and organic systems. The main objective of this step was to evaluate the yield and productivity in FOS, using alternatives for enhancing enzyme activity by means of pressurized fluids and also using low-cost supports for enzyme immobilization, aiming at obtaining a stable biocatalyst to be used for synthesis reactions. Yields of 18 % were achieved using sucrose as substrate in aqueous medium, showing the potential of this procedure, hence suggesting a further optimization step to increase the process yield.

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Acknowledgments

The authors thank CNPq, CAPES, and FAPERGS for the financial support of this work and scholarships.

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Authors and Affiliations

  1. Departamento de Engenharia de Alimentos, URI—Campus de Erechim, Av. Sete de Setembro 1621, Erechim, RS, 99700-000, Brazil

    Graciele de Oliveira Kuhn, Clarissa Dalla Rosa & Marceli Fernandes Silva

  2. Universidade Federal da Fronteira Sul—Campus de Erechim, Av. Dom João Hoffmann, 313, Erechim, RS, 99700-000, Brazil

    Helen Treichel

  3. Departamento de Engenharia Química e Engenharia de Alimentos, Universidade Federal de Santa Catarina (UFSC), Campus Universitário, Bairro Trindade, Caixa Postal 476, Florianópolis, SC, 88040-900, Brazil

    Débora de Oliveira & J. Vladimir Oliveira

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  1. Graciele de Oliveira Kuhn
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  2. Clarissa Dalla Rosa
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  3. Marceli Fernandes Silva
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Correspondence to Helen Treichel.

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de Oliveira Kuhn, G., Rosa, C.D., Silva, M.F. et al. Synthesis of Fructooligosaccharides from Aspergillus niger Commercial Inulinase Immobilized in Montmorillonite Pretreated in Pressurized Propane and LPG. Appl Biochem Biotechnol 169, 750–760 (2013). https://doi.org/10.1007/s12010-012-0007-z

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