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Production of fructooligosaccharides by crude enzyme preparations of β-fructofuranosidase from Aureobasidium pullulans

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Abstract

Fructooligosaccharides (FOS) were produced from sucrose by using crude enzyme preparations of β-fructofuranosidases (FFases) obtained from sucrose-cultured cells of Aureobasidium pullulans DSM 2404. When the preparation mainly consisted of FFase I, that has high transfructosylating activity, the FOS yield was 62%. When the reaction was carried out with additional commercial glucose isomerase (GI) at an activity ratio of FFase and GI of 1:2, the maximum FOS yield reached 69%. This value was higher than those obtained previously using other Aureobasidium spp. (53–59%).

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References

  • Hayashi S, Nonokuchi M, Imada K, Ueno H (1990) Production of a fructosyl-transferring enzyme by Aureobasidium sp. ATCC 20524. J Ind Microbiol 5:395–400

    Article  CAS  Google Scholar 

  • Hayashi S, Nonoguchi M, Takasaki Y, Ueno H, Imada K (1991) Purification and properties of β-fructofuranosidase from Aureobasidium sp. ATCC 20524. J Ind Microbiol 7:251–256

    Article  CAS  Google Scholar 

  • Hidaka H, Hirayama M, Sumi N (1988) A fructooligosaccharide-producing enzyme from Aspergillus niger ATCC 20611. Agric Biol Chem 52:1181–1187

    CAS  Google Scholar 

  • Hirayama M, Sumi N, Hidaka H (1989) Purification and properties of a fructooligosaccharides-producing β-fructofuranosidase from Aspergillus niger ATCC 20611. Agric Biol Chem 53:667–673

    CAS  Google Scholar 

  • Jung KH, Yun JW, Kang KR, Lim JY, Lee JH (1989) Mathematical model for enzymatic production of fructo-oligosaccharides from sucrose. Enzyme Microb Technol 11:491–494

    Article  CAS  Google Scholar 

  • Menne E, Guggenbuhl N, Roberfroid M (2000) Fn-type chicory inulin hydrolysate has a prebiotic effect in humans. J Nutr 130:1197–1199

    PubMed  CAS  Google Scholar 

  • Nakakuki T (2005) Present status and future prospects of functional oligosaccharide development in Japan. J Appl Glycosci 52:267–271

    CAS  Google Scholar 

  • Rao VA (2001) The prebiotic properties of oligofructose at low intake levels. Nutr Res 21:843–848

    Article  CAS  Google Scholar 

  • Shin HT, Baig SY, Lee SW, Suh DS, Kwon ST, Lim YB, Lee JH (2004) Production of fructo-oligosaccharides from molasses by Aureobasidium pullulans cells. Bioresour Technol 93:59–62

    Article  PubMed  CAS  Google Scholar 

  • Takasaki Y (1966) Studies on sugar-isomerizing enzyme production and utilization of glucose isomerase from Streptomyces sp. Agric Biol Chem 30:1247–1253

    CAS  Google Scholar 

  • Tokunaga T (2004) Novel physiological function of fructooligosaccharides. Biofactors 21:89–94

    PubMed  CAS  Google Scholar 

  • Yoshikawa J, Amachi S, Shinoyama H, Fujii T (2006) Multiple β-fructofuranosidase by Aureobasidium pullulans DSM 2404 and their roles in fructooligosaccharide production. FEMS Microbiol Lett 265:159–163

    Article  PubMed  CAS  Google Scholar 

  • Yoshikawa J, Amachi S, Shinoyama H, Fujii T (2007) Purification and some properties of β-fructofuranosidase I formed by Aureobasidium pullulans DSM 2404. J Biosci Bioeng 103:491–493

    Article  PubMed  CAS  Google Scholar 

  • Yun JW, Noh JS, Lee MG, Song SK (1993) Production of fructo-oligosaccharides by the mixed-enzyme system of fructosyltransferase and glucose isomerase. J Korean Inst Chem Eng (in Korean with English abstract) 31:846–851

    CAS  Google Scholar 

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Acknowledgements

We would like to thank Godo Shusei Co., LTD. for providing glucose isomerase (GODO AGI-S).

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Author notes

  1. Jun Yoshikawa

    Present address: Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), AIST Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8565, Japan

Authors and Affiliations

  1. Department of Advanced Bioresources Science, Graduate School of Science and Technology, Chiba University, 648 Matsudo, Matsudo-City, Chiba, 271-8510, Japan

    Jun Yoshikawa, Seigo Amachi, Hirofumi Shinoyama & Takaaki Fujii

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  1. Jun Yoshikawa
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  2. Seigo Amachi
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  3. Hirofumi Shinoyama
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  4. Takaaki Fujii
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Correspondence to Takaaki Fujii.

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Yoshikawa, J., Amachi, S., Shinoyama, H. et al. Production of fructooligosaccharides by crude enzyme preparations of β-fructofuranosidase from Aureobasidium pullulans . Biotechnol Lett 30, 535–539 (2008). https://doi.org/10.1007/s10529-007-9568-2

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  • DOI: https://doi.org/10.1007/s10529-007-9568-2

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