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Hydrolytic Enzyme of Cellulose for Complex Formulation Applied Research

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Abstract

To improve the enzymatic hydrolytic efficiency and reduce the supplementation of enzymes, the mixture designed experimental approach was used to optimize the composition of enzyme mixture and promote the hydrolysis of ball-milled corn stover. From the experimental results, a synergistic effect was found when combinations of the three enzymes, two kinds of cellulases and a kind of xylanase, were used. The optimal hydrolysis of pretreated corn stover accorded with enzymes activity ration of FPU/CMCase/β-glucosidase/xylanase = 4.4:1:75:829, and the hydrolysis efficiency of corn stover increased significantly compared with using individual enzyme. The results indicated that the mixture design experiment could be an effective tool for optimized enzyme mixture for lignocellulose hydrolysis.

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Acknowledgements

This work was financially supported by China Petroleum & Chemical Corporation (No. 207035), National Natural Science Foundation of China (No. 20876078), the Key Program of National Natural Science Foundation of China (No. 20936002), United Foundation of NSFC and Guangdong Province (No. U0733001), National Hi-tech Research and Development Program of China (No. 2009AA02Z08) and National Basic Research Program of China (No. 2007CB707805).

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Authors and Affiliations

  1. State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing, 210009, People’s Republic of China

    Zeng-Xiang Lin, Xiao-Jun Ji, Jing-Wen Chen & He Huang

  2. Department of Applied Chemistry, Nanjing University of Technology, No. 5 Xinmofan Road, Nanjing, 210009, People’s Republic of China

    Hong-Man Zhang

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  1. Zeng-Xiang Lin
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Correspondence to He Huang.

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Lin, ZX., Zhang, HM., Ji, XJ. et al. Hydrolytic Enzyme of Cellulose for Complex Formulation Applied Research. Appl Biochem Biotechnol 164, 23–33 (2011). https://doi.org/10.1007/s12010-010-9111-0

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