Skip to main content

Advertisement

SpringerLink
Log in

Applications of Loop-Mediated Isothermal DNA Amplification

  • Published:
Applied Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

During the last 10 years, with the development of loop-mediated isothermal amplification (LAMP) method, it has been widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency, and outstanding specificity. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA. Expensive equipment are not necessary to acquire a high level of precision, and there are fewer preparation steps compared to conventional PCR and real-time PCR assays. This paper briefly summarized the applications of LAMP method in pathogenic microorganisms, genetically modified ingredients, tumor detection, and embryo sex identification.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Notomi, T., Okayama, H., & Masubuchi, H. (2000). Nucleic Acids Research, 28, e63.

    Article  CAS  Google Scholar 

  2. Ushikubo, H. (2004). Uirusu, 54, 107–112.

    Article  CAS  Google Scholar 

  3. Hara-Kudo, Y., Yoshino, M., Kojima, T., & Ikedo, M. (2005). FEMS Microbiology Letters, 253, 155–161.

    Article  CAS  Google Scholar 

  4. Hara-Kudo, Y., Nemoto, J., Ohtsuka, K., Segawa, Y., Takatori, K., Kojima, T., et al. (2007). Journal of Medical Microbiology, 56, 398–406.

    Article  CAS  Google Scholar 

  5. Yamazaki, W., Taguchi, M., Ishibashi, M., Kitazato, M., Nukina, M., Misawa, N., et al. (2008). Journal of Medical Microbiology, 57, 444–451.

    Article  CAS  Google Scholar 

  6. Yamazaki, W., Taguchi, M., Kawai, T., Kawatsu, K., Sakata, J., Inoue, K., et al. (2009). Applied and Environmental Microbiology, 75, 1597–1603.

    Article  CAS  Google Scholar 

  7. Maruyama, F., Kenzaka, T., Yamaguchi, N., Tani, K., & Nasu, M. (2003). Applied and Environmental Microbiology, 69, 5023–5028.

    Article  CAS  Google Scholar 

  8. Osawa, R., Yoshida, A., Masakiyo, Y., Nagashima, S., Ansai, T., Watari, H., et al. (2007). Oral Microbiology and Immunology, 22, 252–259.

    Article  CAS  Google Scholar 

  9. Iwamoto, T., Sonobe, T., & Hayashi, K. (2003). Journal of Clinical Microbiology, 41, 2616–2622.

    Article  CAS  Google Scholar 

  10. Seki, M., Yamashita, Y., Torigoe, H., Tsuda, H., Sato, S., & Maeno, M. (2005). Journal of Clinical Microbiology, 43, 1581–1586.

    Article  CAS  Google Scholar 

  11. Savan, R., Igarashi, A., Matsuoka, S., & Sakai, M. (2004). Applied and Environmental Microbiology, 70, 621–624.

    Article  CAS  Google Scholar 

  12. Yeh, H. Y., Shoesnaker, C. A., & Klesiue, P. H. (2005). Journal of Microbiological Methods, 63, 36–44.

    Article  CAS  Google Scholar 

  13. Misawa, Y., Yoshida, A., Saito, R., Yoshida, H., Okuzumi, K., Ito, N., et al. (2007). Journal of Infection and Chemotherapy, 13, 134–140.

    Article  CAS  Google Scholar 

  14. Qiao, Y. M., Guo, Y. C., Zhang, X. E., Zhou, Y. F., Zhang, Z. P., Wei, H. P., et al. (2007). Biotechnology Letters, 29, 1939–1946.

    Article  CAS  Google Scholar 

  15. Savan, R., Kono, T., Itami, T., & Sakai, M. (2005). Journal of Fish Diseases, 28, 573–581.

    Article  CAS  Google Scholar 

  16. Fukuta, S., Kato, S., Yoshida, K., Mizukami, Y., Ishida, A., Ueda, J., et al. (2003). Journal of Virological Methods, 12, 35–40.

    Article  Google Scholar 

  17. Qu, G. G., Fu, S. J., Shen, N., Wang, J. L., Xiao, Y. Q., Guan, Y., et al. (2010). Journal of Applied Animal Research, 37, 113–116.

    CAS  Google Scholar 

  18. Ihira, M., Akimoto, S., Miyake, F., Fujita, A., Sugata, K., Suga, S., et al. (2007). Journal of Clinical Virology, 39, 22–26.

    Article  CAS  Google Scholar 

  19. Ihira, M., Yoshikawa, T., Enomoto, Y., Akimoto, S., Ohashi, M., Suga, S., et al. (2004). Journal of Clinical Microbiology, 42, 140–145.

    Article  CAS  Google Scholar 

  20. Yoshikawa, T., Ihira, M., & Akimoto, S. (2004). Journal of Clinical Microbiology, 42, 1348–1352.

    Article  CAS  Google Scholar 

  21. Kaneko, H., Iida, T., Aoki, K., Ohno, S., & Suzutani, T. (2005). Journal of Clinical Microbiology, 43, 3290–3296.

    Article  CAS  Google Scholar 

  22. Poon, L. L., Leung, C. S., Chan, K. H., Lee, J. H., Yuen, K. Y., Guan, Y., et al. (2005). Journal of Clinical Microbiology, 43, 427–430.

    Article  CAS  Google Scholar 

  23. Chen, Q., Li, J., Fang, X. E., & Xiong, W. (2009). Intervirology, 52, 86–91.

    Article  CAS  Google Scholar 

  24. Fukuta, S., Iida, T., Mizukami, Y., Ishida, A., Ueda, J., Kanbe, M., et al. (2003). Archives of Virology, 148, 1713–1720.

    Article  CAS  Google Scholar 

  25. Imai, M., Ninomiya, A., Minekawa, H., Notomi, T., Ishizaki, T., Tashiro, M., et al. (2006). Vaccine, 24, 6679–6682.

    Article  CAS  Google Scholar 

  26. Imai, M., Ninomiya, A., Minekawa, H., Notomi, T., Ishizaki, T., Van-Tu, P., et al. (2007). Journal of Virological Methods, 141, 173–180.

    Article  CAS  Google Scholar 

  27. Kiatpathomchai, W., Jareonram, W., Jitrapakdee, S., & Flegel, T. W. (2007). Journal of Virological Methods, 146, 125–128.

    Article  CAS  Google Scholar 

  28. Endo, S., Komori, T., Ricci, G., Sano, A., Yokoyama, K., Ohori, A., et al. (2004). FEMS Microbiology Letters, 234, 93–97.

    Article  CAS  Google Scholar 

  29. Tatibana, B. T., Sano, A., Uno, J., Kamei, K., Igarashi, T., Mikami, Y., et al. (2009). Journal of Clinical Laboratory Analysis, 23, 139–143.

    Article  CAS  Google Scholar 

  30. Kuboki, N., Inoue, N., Sakurai, T., Di Cello, F., Grab, D. J., Suzuki, H., et al. (2003). Journal of Clinical Microbiology, 41, 5517–5524.

    Article  CAS  Google Scholar 

  31. Thekisoe, O. M., Inoue, N., Kuboki, N., Tuntasuvan, D., Bunnoy, W., Borisutsuwan, S., et al. (2005). Veterinary Parasitology, 130, 327–330.

    Article  CAS  Google Scholar 

  32. Thekisoe, O. M., Kuboki, N., Nambota, A., Fujisaki, K., Sugimoto, C., Igarashi, I., et al. (2007). Acta Tropica, 102, 182–189.

    Article  CAS  Google Scholar 

  33. Han, E. T., Watanabe, R., Sattabongkot, J., Khuntirat, B., Sirichaisinthop, J., Iriko, H., et al. (2007). Journal of Clinical Microbiology, 45, 2521–2528.

    Article  CAS  Google Scholar 

  34. Alhassan, A., Thekisoe, O. M., Yokoyama, N., Inoue, N., Motloang, M. Y., Mbati, P. A., et al. (2007). Veterinary Parasitology, 143, 155–160.

    Article  Google Scholar 

  35. Ikadai, H., Tanaka, H., Shibahara, N., Matsuu, A., Uechi, M., Itoh, N., et al. (2004). Journal of Clinical Microbiology, 42, 2465–2469.

    Article  CAS  Google Scholar 

  36. Karanis, P., Thekisoe, O., Kiouptsi, K., Ongerth, J., Igarashi, I., & Inoue, N. (2007). Applied and Environmental Microbiology, 73, 5660–5662.

    Article  CAS  Google Scholar 

  37. El-Matbouli, M., & Soliman, H. (2005). Journal of Fish Diseases, 28, 549–557.

    Article  CAS  Google Scholar 

  38. Saito, R., Misawa, Y., Moriya, K., Koike, K., Ubukata, K., & Okamura, N. (2005). Journal of Medical Microbiology, 54, 1037–1041.

    Article  CAS  Google Scholar 

  39. Kawai, Y., Miyashita, N., Kishi, F., Tabuchi, M., Oda, K., Yamaguchi, T., et al. (2009). European Journal of Clinical Microbiology & Infectious Diseases, 28, 801–805.

    Article  CAS  Google Scholar 

  40. Fukuta, S., Mizukami, Y., Ishida, A., Ueda, J., Hasegawa, M., Hayashi, I., et al. (2004). European Food Research and Technology, 218, 496–500.

    Article  CAS  Google Scholar 

  41. Lee, D., La Mura, M., Allnutt, T. R., & Powell, W. (2009). BMC Biotechnology, 9, 7.

    Article  Google Scholar 

  42. Guan, X. Y., Guo, J. C., Shen, P., Yang, L. T., & Zhang, D. B. (2010). Food Analytical Methods. doi:10.1007/s12161-010-9132-x.

    Google Scholar 

  43. Hirayama, H., Kageyama, S., Moriyasu, S., Sawai, K., Onoe, S., Takahashi, Y., et al. (2004). Theriogenology, 62, 887–896.

    Article  CAS  Google Scholar 

  44. Hirayama, H., Kageyama, S., Takahashi, Y., Moriyasu, S., Sawai, K., Onoe, S., et al. (2006). Theriogenology, 66, 1249–1256.

    Article  CAS  Google Scholar 

  45. Horibe, D., Ochiai, T., Shimada, H., Tomonaga, T., Nomura, F., Gun, M., et al. (2006). International Journal of Cancer, 120, 1063–1069.

    Article  Google Scholar 

Download references

Acknowledgments

The authors gratefully acknowledge Miss Shirley Wong for the critical reading of the manuscript. This study was financially supported by the Shandong Science and Technology Development Project of People’s Republic of China (2009GG10009001).

Author information

Authors and Affiliations

  1. Key Laboratory of Preventive Veterinary Medicine and Animal Biotechnology, Binzhou Animal Husbandry and Veterinary Research Academy, Binzhou, 256600, People’s Republic of China

    Shijun Fu, Guanggang Qu, Shijin Guo, Songlin Zhang, Sanyang Gao & Zhiqiang Shen

  2. Shandong Lvdu Ante Veterinary Drug Industry Co., Ltd, Binzhou, 256600, People’s Republic of China

    Shijun Fu, Shijin Guo, Lin Ma & Zhiqiang Shen

  3. Shandong Lvdu Biological Technology Co., Ltd, Binzhou, 256600, People’s Republic of China

    Guanggang Qu, Na Zhang, Sanyang Gao & Zhiqiang Shen

Authors
  1. Shijun Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Guanggang Qu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Shijin Guo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Lin Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Na Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Songlin Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sanyang Gao
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Zhiqiang Shen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shijun Fu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Fu, S., Qu, G., Guo, S. et al. Applications of Loop-Mediated Isothermal DNA Amplification. Appl Biochem Biotechnol 163, 845–850 (2011). https://doi.org/10.1007/s12010-010-9088-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-010-9088-8

Keywords

Search

Navigation