Summary.
A rapid and simple procedure is described to detect the genomic RNA molecule of Japanese yam mosaic potyvirus (JYMV). This method, named RT-LAMP, allows direct detection of RNA from infected plants without careful RNA extraction, rapid thermal cycling and gel electrophoresis. RT-LAMP was successfully applied to leaves, propagules and roots of Japanese yam infected with JYMV. One of the characteristics of the RT-LAMP method is its ability to synthesize an extremely large amount of DNA. Accordingly, a large amount of by-product, pyrophospate ion, is produced yielding a white precipitate of magnesium pyrophosphate in the reaction mixture. The presence or absence of this white precipitate allows easy detection of the amplification of JYMV genomic RNA without gel electrophoresis.
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Received January 20, 2003; accepted April 16, 2003 Published online July 2, 2003
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Fukuta, S., Iida, T., Mizukami, Y. et al. Detection of Japanese yam mosaic virus by RT-LAMP. Arch Virol 148, 1713–1720 (2003). https://doi.org/10.1007/s00705-003-0134-5
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DOI: https://doi.org/10.1007/s00705-003-0134-5