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Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis spores

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Abstract

A loop-mediated isothermal amplification (LAMP) assay system was employed for detecting Bacillus anthracis spores in pure cultures as well as in various simulated powder samples. The specificity of the designed LAMP primer sets was validated by assaying 13 B. anthracis strains and 33 non-B. anthracis species. The detection limits of the LAMP assay were 10 spores/tube for pure cultures and 100 spores/2 mg powder for simulated powder samples. The results show that the LAMP protocol is a promising method for detecting B. anthracis.

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Acknowledgements

This work was supported by the Chinese Academy of Sciences. We thank Dr. Zhiming Yuan for providing Bacillus cereus strains.

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Authors and Affiliations

  1. State Key Laboratory of Virology, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, 430071, China

    Yan-Mei Qiao, Yong-Chao Guo, Xian-En Zhang, Ya-Feng Zhou, Zhi-Ping Zhang, Hong-Ping Wei & Dian-Bing Wang

  2. Graduate School, Chinese Academy of Sciences, Beijing, 100049, China

    Yan-Mei Qiao

  3. The Institute of Microbiology & Epidemiology, Academy of Military Medical Sciences, Beijing, 100071, China

    Rui-Fu Yang

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  1. Yan-Mei Qiao
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Correspondence to Xian-En Zhang.

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Qiao, YM., Guo, YC., Zhang, XE. et al. Loop-mediated isothermal amplification for rapid detection of Bacillus anthracis spores. Biotechnol Lett 29, 1939–1946 (2007). https://doi.org/10.1007/s10529-007-9472-9

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  • DOI: https://doi.org/10.1007/s10529-007-9472-9

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