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Metabolic Effects of the Initial Glucose Concentration on Microbial Production of Hyaluronic Acid

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Abstract

The objective of the present work was to evaluate the metabolic effects induced by the initial glucose concentration (IGC) on the cultivation of Streptococcus zooepidemicus for the production of hyaluronic acid (HA). These effects were monitored along non-controlled pH cultivations, carried out in 250-mL Erlenmeyer flasks (natural aeration) and in a 3-L bioreactor (forced aeration) as well. Effects of the IGC were observed with focus on the main metabolites, cell growth, production, and average molecular weight of HA. The absence of glucose resulted in a mixed acid metabolism independent of the oxygen supply, while, for IGCs ranging from 5 to 90 g L-1, the homolactic metabolism was prevalent. The IGC had no influence on the amounts of either biomass or HA produced in the cultivations carried out in flasks; however, cultivations in 3-L bioreactor were found to be strongly dependent on it. The highest concentration of HA (1.21 g L-1) was obtained from 25 g L-1 IGC, the only cultivation where the conversion of glucose to HA was higher than the one of glucose to biomass. Average molecular weight of HA increased concomitant with the IGC, independently of aeration; nevertheless, it decreased along cultivation under forced aeration, due to the shear imparted by stirring.

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Acknowledgements

The authors acknowledge CNPq and FAPESP for the financial support, Investiga Group (Campinas, São Paulo, Brazil) for the maintenance of the bacteria culture, Laboratory of Bioseparations for the availability of High Performance Liquid Chromatography system, and Angela Klatil Ribeiro for the language review.

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  1. Laboratory of Development of Biotechnological Processes School of Chemical Engineering, University of Campinas-UNICAMP, P.O. Box 6066, 13083-970, Campinas, SP, Brazil

    Aline Mara Barbosa Pires & Maria Helena A. Santana

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  1. Aline Mara Barbosa Pires
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  2. Maria Helena A. Santana
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Correspondence to Aline Mara Barbosa Pires.

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Pires, A.M.B., Santana, M.H.A. Metabolic Effects of the Initial Glucose Concentration on Microbial Production of Hyaluronic Acid. Appl Biochem Biotechnol 162, 1751–1761 (2010). https://doi.org/10.1007/s12010-010-8956-6

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