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Influence of glucose fermentation on CO2 assimilation to acetate in homoacetogen Blautia coccoides GA-1

  • Fermentation, Cell Culture and Bioengineering
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

Fermentation of glucose influences CO2 assimilation to acetate in homoacetogens. Blautia coccoides was investigated for a better understanding of the metabolic characteristics of homoacetogens in mixotrophic cultures. Batch cultures of the strain with H2/CO2 as a sole carbon source reached an acetate yield of 5.32 g/g dry cell weight after 240 h of incubation. Autotrophic metabolism was inhibited as glucose was added into the culture: the higher the glucose concentration the lower the autotrophic ability of the bacterium. Autotrophy was inhibited by high glucose concentration, probably due to the competition for coenzyme A between the Embden-Meyerhof-Parnas pathway and the Wood-Ljungdahl carbon fixation pathway, the energy (adenosine triphosphate) allocation for synthesis of cell carbon and reduction of CO2, in combination with the low pH caused by the accumulation of acetate.

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Acknowledgments

This work was supported financially by National Natural Science Foundation of China (Grant No. 51178136), and the State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (Grant No. 2013DX11).

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Authors and Affiliations

  1. School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin, 150090, China

    Chong Liu, Jianzheng Li, Yupeng Zhang, Antwi Philip, En Shi, Xue Chi & Jia Meng

  2. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin, 150090, China

    Jianzheng Li

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  1. Chong Liu
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Correspondence to Jianzheng Li.

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Liu, C., Li, J., Zhang, Y. et al. Influence of glucose fermentation on CO2 assimilation to acetate in homoacetogen Blautia coccoides GA-1. J Ind Microbiol Biotechnol 42, 1217–1224 (2015). https://doi.org/10.1007/s10295-015-1646-1

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  • DOI: https://doi.org/10.1007/s10295-015-1646-1

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