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Potential Application of Alkaline Pectinase from Bacillus subtilis SS in Pulp and Paper Industry

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Abstract

Pectinase production from Bacillus subtilis SS was optimized under solid-state fermentation (5,943 U/g of dry bacterial bran). The pectinase produced was stable in neutral to alkaline pH range at 70 °C; therefore, the suitability of this pectinase in pulp and paper industry was investigated. The enzyme pretreatment process was optimized, and a pectinase dose of 5 IU/g of oven-dried pulp (10% consistency) at pH 9.5 temperature 70 °C after 150 min of treatment gave the best pretreatment to the pulp. An increase of 4.3% in brightness along with an increase of 14.8 and 65.3% in whiteness and fluorescence, respectively, whereas a 15% decrease in the yellowness of the pretreated pulp were observed. There was a 5.85% reduction in kappa number and 6.1% reduction in permanganate number along with a reduction in the chemical oxygen demand value. Significant characteristics showed by pectinase open new possibilities of application of this cellulase-free enzyme in the pulp and paper industry by reducing the negative environmental impact of chemicals apart from improving the properties of paper.

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Acknowledgment

The authors thank Ballarpur Industries (BILT), Haryana, India, for providing kraft pulp and laboratory facilities. Sonia Ahlawat greatly acknowledges the financial assistance provided by Kurukshetra University, Kurukshetra, in the form of University Research Scholarship during the course of the investigation.

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Authors and Affiliations

  1. Department of Biotechnology, Kurukshetra University, Kurukshetra, 136119, India

    Sonia Ahlawat, R. P. Mandhan, Saurabh Sudha Dhiman, Rakesh Kumar & Jitender Sharma

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  1. Sonia Ahlawat
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  2. R. P. Mandhan
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  3. Saurabh Sudha Dhiman
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  4. Rakesh Kumar
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  5. Jitender Sharma
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Correspondence to Jitender Sharma.

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Ahlawat, S., Mandhan, R.P., Dhiman, S.S. et al. Potential Application of Alkaline Pectinase from Bacillus subtilis SS in Pulp and Paper Industry. Appl Biochem Biotechnol 149, 287–293 (2008). https://doi.org/10.1007/s12010-007-8096-9

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  • DOI: https://doi.org/10.1007/s12010-007-8096-9

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