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Improvement of Pineapple Leaf Fiber Quality by Pectinase Produced from Newly Isolated Bacillus subtilis subsp. inaquosorum P4-1

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Abstract

This research aims to optimize the conditions for improvement of pineapple leaf fiber quality using pectinase from selected bacterial strains compared with the commercial ones. First, Bacillus subtilis subsp. inaquosorum P4-1 was selected for its highest capability of pectinase production (19.59±0.47 U/ml) obtained from natural sources in Thailand. After that, the response surface methodology was applied for the optimization of cultivation condition to reach highest pectinase production. Highest pectinase activity at 59.86 U/ml was obtained when B. subtilis P4-1 was cultivated at 150 rpm, 35 °C in the liquid media containing 18.65 g/l citrus pectin, 4.12 g/l Monosodium-L-glutamate, 2.4 g/l KH2PO4, 0.5 g/l MgSO4·7H2O, 0.8 g/l Na2HPO4, 3 g/l (NH4)2SO4, 0.2 g/l CaCl2. To improve the pineapple leaf fiber quality, the pineapple leaves were first fed into the fiber extraction machine to obtain pineapple leaf fiber as raw materials to be used in this experiment. The 3 commercially available pectinase in Thailand: Pectinex Ultra Tropical, Pectinex Ultra SP-L and Scourzyme L, were compared and the results showed that Pectinex Ultra SP-L produced pineapple leaf fiber with the best quality at enzyme:fiber ratio of 1:10 in combination with 0.75 % tergitol surfactant after 1 day of soaking period. The same conditions were then applied for the improvement of pineapple leaf fiber quality using pectinase from selected bacterial B. subtilis P4-1. The resulting fibers showed no significant difference with that from the commercial pectinase after the sensory test.

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Acknowledgment

Our appreciation goes to Kasetsart University Research and Development Institute (KURDI) for their support and endorsement of this research. Our appreciation also goes to the natural fiber producer group, Ban Kha District, Ratchaburi, for their sponsorship of the fibers from the pineapple leaves that were used throughout the study.

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Authors and Affiliations

  1. Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, Bangkok, 10900, Thailand

    Pilanee Vaithanomsat, Chanaporn Trakunjae, Jiraporn Meelaksana, Antika Boondaeng, Waraporn Apiwatanapiwat, Pornpimon Janchai, Jirachaya Boonyarit & Rungsima Chollakup

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  1. Pilanee Vaithanomsat
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  2. Chanaporn Trakunjae
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  3. Jiraporn Meelaksana
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  4. Antika Boondaeng
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  5. Waraporn Apiwatanapiwat
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  7. Jirachaya Boonyarit
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  8. Rungsima Chollakup
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Correspondence to Rungsima Chollakup.

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Vaithanomsat, P., Trakunjae, C., Meelaksana, J. et al. Improvement of Pineapple Leaf Fiber Quality by Pectinase Produced from Newly Isolated Bacillus subtilis subsp. inaquosorum P4-1. Fibers Polym 23, 576–588 (2022). https://doi.org/10.1007/s12221-021-0120-0

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  • DOI: https://doi.org/10.1007/s12221-021-0120-0

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