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QTL analysis of agronomic traits in recombinant inbred lines of sunflower under partial irrigation

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Abstract

The objective of the present research was to map QTLs associated with agronomic traits such as days from sowing to flowering, plant height, yield and leaf-related traits in a population of recombinant inbred lines (RILs) of sunflower (Helianthus annuus). Two field experiments were conducted with well-irrigated and partially irrigated conditions in randomized complete block design with three replications. A map with 304 AFLP and 191 SSR markers with a mean density of 1 marker per 3.7 cM was used to identify QTLs related to the studied traits. The difference among RILs was significant for all studied traits in both conditions. Three to seven QTLs were found for each studied trait in both conditions. The percentage of phenotypic variance (R 2) explained by QTLs ranged from 4 to 49%. Three to six QTLs were found for each yield-related trait in both conditions. The most important QTL for grain yield per plant on linkage group 13 (GYP-P-13-1) under partial-irrigated condition controls 49% of phenotypic variance (R 2). The most important QTL for 1,000-grain weight (TGW-P-11-1) was identified on linkage group 11. Favorable alleles for this QTL come from RHA266. The major QTL for days from sowing to flowering (DSF-P-14-1) were observed on linkage group 14 and explained 38% of the phenotypic variance. The positive alleles for this QTL come from RHA266. The major QTL for HD (HD-P-13-1) was also identified on linkage group 13 and explained 37% of the phenotypic variance. Both parents (PAC2 and RHA266) contributed to QTLs controlling leaf-related traits in both conditions. Common QTL for leaf area at flowering (LAF-P-12-1, LAF-W-12-1) was detected in linkage group 12. The results emphasise the importance of the role of linkage groups 2, 10 and 13 for studied traits. Genomic regions on the linkage groups 9 and 12 are specific for QTLs of leaf-related traits in sunflower.

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Abbreviations

QTL:

Quantitative trait locus

CIM:

Composite interval mapping

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Authors and Affiliations

  1. Laboratoire de Symbiose et Pathologie des Plantes (SP2), IFR 40, INP-ENSAT, 18 Chemin de Borde Rouge, BP 32607, 31326, Castanet Tolosan, France

    P. Haddadi & A. Sarrafi

  2. Agronomy and Plant Breeding Department, Faculty of Agriculture, University of Tehran, Karaj, Iran

    P. Haddadi, B. Yazdi-samadi, M. R. Naghavi & A. Kalantari

  3. Agrosystèmes et Développement Territorial, UMR 1248, INRA, INP-ENSAT, 31326, Castanet Tolosan, France

    P. Maury

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  1. P. Haddadi
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  2. B. Yazdi-samadi
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  3. M. R. Naghavi
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  4. A. Kalantari
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  5. P. Maury
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  6. A. Sarrafi
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Correspondence to A. Sarrafi.

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Haddadi, P., Yazdi-samadi, B., Naghavi, M.R. et al. QTL analysis of agronomic traits in recombinant inbred lines of sunflower under partial irrigation. Plant Biotechnol Rep 5, 135–146 (2011). https://doi.org/10.1007/s11816-011-0164-5

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  • DOI: https://doi.org/10.1007/s11816-011-0164-5

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