Abstract
Solvent-free microwave extraction (SFME) of polyphenols and flavonoids from mandarin (Citrus deliciosa Tenore) leaves was investigated. A face central composite design (FCCD) through response surface methodology (RSM) was applied to study the effects of extraction time (30–90 sec), microwave irradiation power (250–350 W) and solid mass (2.5–7.5 g), and to optimize the extraction process. The optimum conditions were: extraction time, 53.155 sec; microwave power, 339.190W; and solid mass, 2.500 g. Under the optimum conditions, 0.8610mg-GAE/g-DL of total phenolic content (TPC) and 0.2440mg-CE/g-DL of total flavonoid content (TFC) were extracted. The antioxidant activity of the extracts was assessed by cupric ion reducing antioxidant capacity (CUPRAC), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) methods, respectively. Antioxidant values were expressed as mg trolox equivalent antioxidant capacity per gram of dried leaf (mg-TEAC/g-DL). CUPRAC values were highly correlated with both TPC and TFC (r=0.9282/0.8842, P=0.05) in the extracts, whilst DPPH (r=0.7717/0.7435, P=0.05) and ABTS (r=0.6814/0.7072, P=0.05) were relatively less correlated with the same responses.
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References
B. B. Li, B. Smith and Md. M. Hossain, Sep. Purif. Technol., 48, 182 (2006).
A. Bocco, M.E. Cuve, H. Richard and C. Berset, J. Agric. Food Chem., 46, 2123 (1998).
F. Dahmoune, L. Boulekbache, K. Moussi, O. Aoun, G. Spigno and K. Madani, Ind. Crop Prod., 50, 77 (2013).
Y. Sun, D. Liu, J. Chen, X. Ye and D. Yu, Ultrason. Sonochem., 18, 243 (2011).
N. Balasundram, K. Sundram and S. Samman, Food Chem., 99, 191 (2006).
K. Hayat, S. Hussain, S. Abbas, U. Farooq, B. Ding, S. Xia, C. Jia, X. Zhang and W. Xia, Sep. Purif. Technol., 70, 63 (2009).
J. Londoño-Londoño, V.R.D. Lima, O. Lara, A. Gil, T. B. C. Pasa, G. J. Arango and J. R. R. Pineda, Food Chem., 119, 81 (2010).
M.K. Khan, M. Abert-Vian, A. S. Fabiano-Tixier, O. Dangles and F. Chemat, Food Chem., 119, 851 (2010).
B. B. Li, B. Smith and Md. M. Hossain, Sep. Purif. Technol., 48, 189 (2006).
M. L. Lota, D.D.R. Serra, F. Tomi and J. Casanova, Biochem. Syst. Ecol., 29, 77 (2001).
Y.Q. Ma, J. C. Chen, D. H. Liu and X.Q. Ye, J. Food Sci., 73, 115 (2008).
G. H. Xu, J. C. Chen, D. H. Liu, Y. H. Zhang, P. Jiang and X.Q. Ye, J. Food Sci., 73, C11 (2008).
F. Le Floch, M.T. Tena, A. Rios and M. Valcarcel, Talanta, 46, 1123 (1998).
R. Japón-Lujàn, J.M. Luque-Rodríguez, and M.D. Luque De Castro, Anal. Bioanal. Chem., 385, 753 (2006).
D. Baycin, E. Altiok, S. Ülkü and O. Bayraktar, J. Agric. Food Chem., 55, 1227 (2007).
X. Pan, G. Niu and H. Liu, Chem. Eng. Process., 42, 129 (2003).
H. Li, Z. Deng, T. Wu, R. Liu, S. Loewen and R. Tsao, Food Chem., 130, 928 (2012).
N. Hong, V.A. Yaylayan, G. S.V. Raghavan, J.R. J. Paré and J.M.R. Bélanger, Nat. Prod. Lett., 15, 197 (2001).
A. Singh, K. Sabally, S. Kubow, D. J. Donnelly, Y. Gariepy, V. Orsat and G. S. V. Raghavan, Mol., 16, 2218 (2011).
T.S. Ballard, P. Mallikarjunan, K. Zhou and S. O’Keefe, Food Chem., 120, 1185 (2010).
Zill-e-Huma, M.A. Vian, J.F. Maingonnat and F. Chemat, J. Chromatogr. A, 1216, 7700 (2009).
B. Bayramoglu, S. Sahin and G. Sumnu, J. Food Eng., 88, 535 (2008).
O.O. Okoh, A.P. Sadimenko and A. J. Afolayan, Afr. J. Biotechnol., 10, 4207 (2011).
M.E. Lucchesi, J. Smadja, S. Bradshaw, W. Louw and F. Chemat, J. Food Eng., 79, 1079 (2007).
B. Uysal, F. Sozmen, O. Aktas, B.S. Oksal and E.O. Kose, Int. J. Food Sci. Technol., 46, 1455 (2011).
X. Chen, Y. Zhang, Y. Zu, Y. Fu and W. Wang, LWT-Food Sci. Technol., 44, 2047 (2011).
X. J. Li, W. Wang, M. Luo, C.Y. Li, Y. G. Zu, P. S. Mu and Y. J. Fu, Food Chem., 133, 437 (2012).
S. Périno-Issartier, Zill-e-Huma, M. Abert-Vian and F. Chemat, Food Bioprocess Technol., 4, 1020 (2011).
T. Michel, E. Destandau and C. Elfakir, Food Chem., 126, 1380 (2011).
N. Saatci and B. Y. Mur, J. Plant Nutr., 23, 1745 (2000).
P.K. Wilmsen, D. S. Spada and M. Salvador, J. Agric. Food Chem., 53, 4757 (2005).
K. Hayat, X. Zhang, U. Farooq, S. Abbas, S. Xia, C. Jia, F. Zhong and J. Zhang, Food Chem., 123, 423 (2010).
K. Hayat, X. Zhang, H. Chenc, S. Xia, C. Jia and F. Zhong, Sep. Purif. Technol., 73, 371 (2010).
S. I. Kirbaslar and F. G. Kirbaslar, J. Essent. Oil Res., 18, 318 (2006).
L. Pérez, E. Conde and H. Domínguez, Process Biochem., 49, 981 (2014).
N. S. A. Malik and J. M. Bradford, Sci. Hortic., 110, 274 (2006).
S. Sakanaka, Y. Tachibana and Y. Okada, Food Chem., 89, 569 (2005).
J. Yu, L. Wang, R. L. Walzem, E.G. Miller, L. M. Pike and B. S. Patil, J. Agric. Food Chem., 53, 2009 (2005).
S. Sahin and R. Samli, Ultrason. Sonochem., 20, 595 (2013).
M. Bilgin, S. Sahin, M.U. Dramur and M. L. Sevgili, Chem. Eng. Commun., 200, 1197 (2013).
R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang and C. Rice-Evans, Free Radic. Biol. Med., 26, 1231 (1999).
R. Apak, K. Güçlü, M. Özyürek and S.E. Çelik, Microchim. Acta, 160, 413 (2008).
N. R. Draper and J. A. John, Technometrics, 30, 423 (1998).
I.T. Karabegovic, S. S. Stojicevic, D.T. Velickovic, N. C. Nikolic and M. L. Lazic, Sep. Sci. Technol., 49, 416 (2014).
H. Li, X. Wang, P. Li, Y. Li and H. Wang, J. Food Drug Anal., 16, 67 (2008).
H. Li, X. Wang, P. Li, Y. Li and H. Wang, Food Chem., 112, 454 (2009).
Y. Pan, C. He, H. Wang, X. Ji, K. Wang and P. Liu, Food Chem., 121, 497 (2010).
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Şahin, S. A novel technology for extraction of phenolic antioxidants from mandarin (Citrus deliciosa Tenore) leaves: Solvent-free microwave extraction. Korean J. Chem. Eng. 32, 950–957 (2015). https://doi.org/10.1007/s11814-014-0293-y
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DOI: https://doi.org/10.1007/s11814-014-0293-y