Abstract
The effect of mineral addition on arachidonic acid (AA) production by Mortierella alpina 1S-4 was evaluated. At first, the addition of minerals such as sodium, potassium, calcium, and magnesium was examined in flask cultures, and then the addition of phosphorus with the optimal amounts of the minerals was investigated in a 10-L jar-fermenter. As a result, 1.5% soy flour medium with the addition of 0.3% KH2PO4, 0.1% Na2SO4, 0.05% CaCl2·2H2O, and 0.05% MgCl2·6H2O was found to enhance the AA yield 1.7-fold over that without mineral addition. When 1% yeast extract with the above mineral mixture was used, the AA yield was enhanced 1.35-fold over that without minerals. We also verified that an increase in the polar lipid content occurred in the case of only KH2PO4 addition, and that the above-mentioned increase in the AA yield was due to the minerals themselves, not a pH buffer effect.
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References
Gill, I., and R. Valivety, Polyunsaturated Fatty Acids, Part 1: Occurrence, Biological Activities and Application, Trends Biotechnol. 15:401–409 (1997).
Carlson, S.E., S.H. Werkman, J.M. Peeples, R.J. Cooke, and E.A. Tolley, Arachidonic Acid Status Correlates with First Year Growth in Preterm Infants, Proc. Natl. Acad. Sci. USA 90:1073–1077 (1993).
Shinmen, Y., S. Shimizu, K. Akimoto, H. Kawashima, and H. Yamada, Production of Arachidonic Acid by Mortierella Fungi, Appl. Microbiol. Biotechnol. 31:11–16 (1989).
Official Methods and Recommended Practices of the American Oil Chemists’ Society, 4th edn., AOCS Press, Champaign, 1997, Section I, pp. 77.
Aiba, S., A.E. Humphrey, and N.F. Millis, The Characteristics of Biological Material, in Biochemical Engineering, 2nd edn., University of Tokyo Press, 1973, pp. 29–30.
Shimizu, S., H. Kawashima, Y. Shinmen, K. Akimoto, and H. Yamada, Production of Eicosapentaenoic Acid by Mortierella Fungi, J. Am. Oil Chem. Soc. 65:1455–1459 (1988).
Yaguchi, T., S. Tanaka, T. Yokochi, T. Nakahara, and T. Higashihara, Production of High Yield of Docosahexaenoic Acid by Schizochytrium sp. Strain SR21, Ibid.:1431–1434 (1997).
Berkley, W.R., U.S. Patent 08/377766 (1995).
Kyle, D.J., PCT Patent WO92/13086 (1992).
Lindberg, A.M., and G. Mollin, Effects of Temperature and Glucose Supply on the Production of Polyunsaturated Fatty Acids by the Fungus Mortierella alpina CBS343.66 in Fermenter Cultures, Appl. Microbiol. Bioetechol. 39:450–455 (1993).
Chen, H.C., C.C. Chang, and C.X. Chen, Optimization of Arachidonic Acid Production by Mortierella alpina Wuji-H14 Isolate, J. Am. Oil Chem. Soc. 74:569–578 (1997).
Sajbidor, J., S. Dobronova, and M. Certik, Arachidonic Acid Production by Mortierella sp. S-17, Biotechnol. Lett. 12:455–456 (1990).
Hansson, L., M. Dostalek, and B. Sorenby, Production of γ-Linolenic Acid by Fungus Mucor rouxii in Fed-Batch and Continuous Culture, Appl. Microbiol. Biotechnol. 31:223–227 (1989).
Bajpai, P.K., P. Bajpai, and O. Ward, Production of Arachidonic Acid by Mortierella alpina ATCC32222, J. Ind. Microbiol. 8:179–186 (1991).
Jackson, F.M., T.C.M. Fraser, M.A. Smith, C. Lazarus, A.K. Stobart, and G. Griffiths, Biosynthesis of C18 Polyunsaturated Fatty Acids in Microsomal Membrane Preparations from the Filamentous Fungus Mucor circinelloides, Eur. J. Biochem. 252:513–519 (1998).
Byrne, G.S., and O.P. Ward, Effect of Nutrition on Pellet Formation by Rhizopus arrhizus, Biotechnol. Bioeng. 33:912–914 (1989).
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Higashiyama, K., Yaguchi, T., Akimoto, K. et al. Enhancement of arachidonic acid production by Mortierella alpina 1S-4. J Amer Oil Chem Soc 75, 1501–1505 (1998). https://doi.org/10.1007/s11746-998-0085-9
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DOI: https://doi.org/10.1007/s11746-998-0085-9