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Production of 8,11,14,17-cis-eicosatetraenoic acid (20:4ω-3) by a Δ5 and Δ12 desaturase-defective mutant of an arachidonic acid-producing fungus Mortierella alpina 1S-4

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Journal of the American Oil Chemists' Society

Abstract

A Δ5 and Δ12 desaturase-defective mutant of an arachidonic acid-producing fungus, Mortierella alpina 1S-4, produced 8,11,14,17-cis-eicosatetraenoic acid (20:4ω3) intracellularly when grown with linseed oil. Dihomo-γ-linolenic acid was the only C20 polyunsaturated fatty acid (4.9 wt% of total mycelial fatty acids) other than 20:4ω3. AA and 5,8,11,14,17-cis-eicosapentaenoic acid were not detected. The mycelial lipids consisted of 82.2% (by mol) triacylglycerol (TG), 7.1% diacylglycerol, 8.9% phospholipids (PL), and 1.9% free fatty acids. The percentage of 20:4ω3 was higher in PL (30.1%) than in TG (11.6%), and highest in phosphatidylcholine (38.9%). Under the optimal conditions with a 5-L jar fermenter, 20:4ω3 production amounted to 97.4 mg/g dry mycelia with a mycelial yield of 23 g/L on the twelfth day (corresponding to 2.24 g/L medium and 37.1% of total mycelial fatty acids).

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References

  1. Oliw, E.H., H. Sprecher, and M. Hamberg, Biosynthesis of a Novel Prostaglandin, Δ17-PGE1, in the Ram, Acta. Physiol. Scand. 127:45–49 (1986).

    Article  PubMed  CAS  Google Scholar 

  2. Samel, N., I. Järving, M. Lohmus, A. Lopp, G. Kobzar, V. Sadovskaya, T. Välimäe, and Ü. Lille, Identification and Biological Activity of a Novel Natural Prostaglandin, 5,6-Dihydroprostaglandin E3, Prostaglandin 33:137–146 (1987).

    Article  CAS  Google Scholar 

  3. Shimizu, S., H. Kawashima, K. Akimoto, Y. Shinmen, and H. Yamada, Conversion of Linseed Oil to an Eicosapentaenoic Acid-Containing Oil by Mortierella alpina 1S-4 at Low Temperature, Appl. Microbiol. Biotechnol. 32:1–4 (1989).

    Article  CAS  Google Scholar 

  4. Kawashima, H., N. Kamada, E. Sakuradani, S. Jareonkitmongkol, K. Akimoto, and S. Shimizu, Production of 8,11,14,17-Eicosapentaenoic Acid (20:4ω3) by Δ5 Desaturase-Defective Mutants of Mortierella alpina 1S-4, J. Am. Oil Chem. Soc. 74:455–459 (1997).

    Article  CAS  Google Scholar 

  5. Shinmen, Y., S. Shimizu, K. Akimoto, H. Kawashima, and H. Yamada, Production of Arachidonic Acid by Mortierella Fungi: Selection of a Potent Producer and Optimization of Culture Conditions for Large-Scale Production, Appl. Microbiol. Biotechnol. 31:11–16 (1989).

    Article  CAS  Google Scholar 

  6. Jareonkitmongkol, S., E. Sakuradani, and S. Shimizu, A Novel Δ5-Desaturase-Defective Mutant of Mortierella alphina 1S-4 and Its Dihomo-γ-Linolenic Acid Productivity, Appl. Environ. Microbiol. 59:4300–4304 (1993).

    PubMed  CAS  Google Scholar 

  7. Kamada, N., M. Nishihara, Y. Hirano, H. Kawashima, K. Akimoto, and S. Shimizu, Production of Mead Acid by a Δ6 Desaturase Activity-Enhanced Mutant Derived from a Δ12 Desaturase-Defective Mutant of Mortierella alpina 1S-4, in Abstract of Annual Meeting of the Society for Fermentation and Bioengineering, Nippon Seibutukogakukai, Osaka, 1996, p. 100.

    Google Scholar 

  8. Kamada, N., S. Jareonkitmongkol, H. Kawashima, M. Nishihara, and S. Shimizu, Production of cis-8,11-Eicosadienoic Acid by a Δ5 and Δ12 Desaturase-Defective Mutant of Mortierella alpina 1S-4, Nippon Nogeikagaku Kaishi 69 Supplement:311 (1995).

  9. Shimizu, S., S. Jareonkitmongkol, H. Kawashima, K. Akimoto, and H. Yamada, Production of a Novel ω1-Eicosapentaenoic Acid by Mortierella alpina 1S-4 Grown on 1-Hexadecene, Arch. Microbiol. 156:163–166 (1991).

    Article  CAS  Google Scholar 

  10. Folch, J., M. Lees, and G.H.S. Stanley, A Simple Method for Isolation and Purification of Total Lipids from Animal Tissues, J. Biol. Chem. 226:497–509 (1957).

    PubMed  CAS  Google Scholar 

  11. Jareonkitmongkol, S., S. Shimizu, and H. Yamada, Production of an Eicosapentaenoic Acid-Containing Oil by a Δ12 Desaturase-Defective Mutant of Mortierella alpina 1S-4, J. Am. Oil Chem. Soc. 70:119–123 (1993).

    Article  CAS  Google Scholar 

  12. Brenner, R.R., and R.O. Peluffo, Effect of Saturated and Unsaturated Fatty Acids on the Desaturation in vitro of Palmitic, Stearic, Oleic, Linoleic, and Linolenic Acids, J. Biol. Chem. 241:5213–5219 (1966).

    PubMed  CAS  Google Scholar 

  13. Shimizu, S., H. Kawashima, Y. Shinmen, K. Akimoto, and H. Yamada, Production of Eicosapentaenoic Acid by Mortierella Fungi, J. Am. Oil Chem. Soc. 65:1455–1459 (1988).

    CAS  Google Scholar 

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Authors and Affiliations

  1. Institute for Biomedical Research, Suntory Ltd., Shimamoto-cho, 618-000, Osaka, Japan

    Hiroshi Kawashima, Kengo Akimoto & Kyoko Konishi

  2. Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawaoiwakecho, Sakyo-ku, 606-8502, Kyoto, Japan

    Eiji Sakuradani, Nozomu Kamada, Jun Ogawa & Sakayu Shimizu

Authors
  1. Hiroshi Kawashima
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  2. Eiji Sakuradani
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  3. Nozomu Kamada
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  4. Kengo Akimoto
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  5. Kyoko Konishi
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  6. Jun Ogawa
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  7. Sakayu Shimizu
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Correspondence to Sakayu Shimizu.

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Kawashima, H., Sakuradani, E., Kamada, N. et al. Production of 8,11,14,17-cis-eicosatetraenoic acid (20:4ω-3) by a Δ5 and Δ12 desaturase-defective mutant of an arachidonic acid-producing fungus Mortierella alpina 1S-4. J Amer Oil Chem Soc 75, 1495–1500 (1998). https://doi.org/10.1007/s11746-998-0084-x

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  • DOI: https://doi.org/10.1007/s11746-998-0084-x

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