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Production of 8,11,14,17-cis-eicosatetraenoic acid by Δ5 desaturase-defective mutants of an arachidonic acid-producing fungus, Mortierella alpina

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Journal of the American Oil Chemists' Society

Abstract

Δ5 Desaturase-defective mutants of an arachidonic acid-producing fungus, Mortierella alpina 1S-4, accumulate large amounts of 8,11,14,17-cis-eicosatetraenoic acid (20:4ω3) when grown with linseed oil. One of the mutants, the S14 strain, produced 1.65 mg of 20:4ω3 per mL of culture medium (corresponding to 66.0 mg/g dry mycelia and 11.6% of total cellular fatty acids) when grown in a medium containing 1% glucose, 1% yeast extract, and 4% linseed oil methyl ester at 28°C for 2 d, and then at 16°C for 7 d. In a bench-scale fermentation in a 5-L jar fermenter, 20:4ω3 production reached 1.60 g/L of culture medium on the eighth day (corresponding to 77.3 mg/g dry mycelia and 26.0% of total cellular fatty acids). The cellular lipids of the S14 strain comprised 75.8% triacylglycerol (TG), 6.7% diacylglycerol, and 13.3% phospholipids (PL). The percentage of 20:4ω3 was higher in PL than in TG, and highest in phosphatidylcholine (32.6%).

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Author notes

  1. Hiroshi Kawashima & Kengo Akimoto

    Present address: Institute for Biomedical Research, Suntory Ltd., Shimamoto-cho, Mishima-gun, 618, Osaka, Japan

Authors and Affiliations

  1. Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, 606, Kyoto, Japan

    Hiroshi Kawashima, Nozomu Kamada, Eiji Sakuradani, Saeree Jareonkitmongkol, Kengo Akimoto & Sakayu Shimizu

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  1. Hiroshi Kawashima
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  2. Nozomu Kamada
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  3. Eiji Sakuradani
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  4. Saeree Jareonkitmongkol
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  5. Kengo Akimoto
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  6. Sakayu Shimizu
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Kawashima, H., Kamada, N., Sakuradani, E. et al. Production of 8,11,14,17-cis-eicosatetraenoic acid by Δ5 desaturase-defective mutants of an arachidonic acid-producing fungus, Mortierella alpina . J Amer Oil Chem Soc 74, 455–459 (1997). https://doi.org/10.1007/s11746-997-0106-0

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  • DOI: https://doi.org/10.1007/s11746-997-0106-0

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