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Production of an eicosapentaenoic acid-containing oil by a Δ12 desaturase-defective mutant ofMortierella alpina 1S-4

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Journal of the American Oil Chemists’ Society

Abstract

A Δ12 desaturase-defective mutant of an arachidonic acid (AA)-producing fungus,Mortierella alpina 1S-4, converted α-linolenic acid (18:3ω3) to 5(Z),8(Z),11(Z),14(Z),17(Z)-eicosapentaenoic acid (EPA). On submerged cultivation at 20°C for 10 d in a 5-L fermentor containing medium comprising 1% glucose, 1% yeast extract and 3% (vol/vol) linseed oil, EPA production amounted toca. 1 g/L culture broth (64 mg/g dry mycelium), which accounted forca. 20% of the total mycelial fatty acids. AA content was 26 mg/g dry mycelium (0.4 g/L), accounting for 7.8% of the total mycelial fatty acids. The other major mycelial fatty acids were palmitic acid (4.5%), oleic acid (20.4%), linoleic acid (10.0%), 18:3ω3 (20.3%) and lignoceric acid (4.3%). Most of the EPA produced (ca. 90 mol%) was in triglyceride form.

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Authors and Affiliations

  1. Department of Agricultural Chemistry, Kyoto University, Sakyo-ku, 606, Kyoto, Japan

    Saeree Jareonkitmongkol, Sakayu Shimizu & Hideaki Yamada

Authors
  1. Saeree Jareonkitmongkol
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  2. Sakayu Shimizu
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  3. Hideaki Yamada
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Jareonkitmongkol, S., Shimizu, S. & Yamada, H. Production of an eicosapentaenoic acid-containing oil by a Δ12 desaturase-defective mutant ofMortierella alpina 1S-4. J Am Oil Chem Soc 70, 119–123 (1993). https://doi.org/10.1007/BF02542612

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  • DOI: https://doi.org/10.1007/BF02542612

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