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Molecular species of phosphoglycerides in liver microsomes of rats fed a fat-free diet

  • Published:
Lipids

Abstract

The influence of a fat-free diet on the molecular species composition of phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylinositol (PI) of rat liver microsomes was studied by using reversed-phase high-pressure liquid chromatography. In the three phosphoglyceride classes analyzed, the fat-free diet produced a large decrease in the 18:0/20:4n−6 species but less important changes were found in the 16:0/20:4n−6 species. In PC, the most abundant phosphoglyceride class of rat liver microsomes, the fall in the 18:0/20:4n−6 species was counterbalanced mainly by an enhancement in the 16:0/18:1n−9 species although it was not evident in PE. In PI, the decrease in the 18:0/20:4n−6 species was counterbalanced by an increase in the 18:0/20:3n−9 species. Fluorescence polarization measurements of 1,6-diphenyl-1,3,5-hexatriene in liposomes of 16:0/18:1n−9-, 18:0/18:1n−9-, 16:0/20:4n−6-, and 18:0/20:4n−6-PC indicated that the change in the saturated fatty acid in the sn-1 position accompanying the replacement of 20:4n−6 by 18:1n−9 could be very important for a homeoviscous compensation, maintaining the membrane physical properties without large alterations in spite of the essential fatty acid deficiency due to the fat-free diet.

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Abbreviations

DPH:

1,6-Diphenyl-1,3,5-hexatriene

EFA:

essential fatty acids

HPLC:

high-performance liquid chromatography

PC:

phosphatidylcholine

PE:

phosphatidylethanolamine

PI:

phosphatidylinositol

UV:

ultraviolet

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Author notes

  1. Horacio A. Garda & Rodolfo R. Brenner

    Present address: Carrera del Investigador Científico, CONICET, Argentina

Authors and Affiliations

  1. Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP) CONICET-UNLP, Facultad de Ciencias Médicas, Calle 60 y 120, 1900-La, Plata, Argentina

    Horacio A. Garda, Ana M. Bernasconi, M. Alejandra Tricerri & Rodolfo R. Brenner

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  1. Horacio A. Garda
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  2. Ana M. Bernasconi
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  3. M. Alejandra Tricerri
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  4. Rodolfo R. Brenner
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Garda, H.A., Bernasconi, A.M., Tricerri, M.A. et al. Molecular species of phosphoglycerides in liver microsomes of rats fed a fat-free diet. Lipids 32, 507–513 (1997). https://doi.org/10.1007/s11745-997-0065-5

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  • DOI: https://doi.org/10.1007/s11745-997-0065-5

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