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Vaccenic acid (t11–18∶1) is converted to c9,t11-CLA in MCF-7 and SW480 cancer cells

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Lipids

Abstract

The aims of this study were to determine whether vaccenic acid (VA; t11–18∶1) is converted to c9,t11-CLA in human mammary (MCF-7) and colon (SW480) cancer cell lines and whether VA influences cell viability and other CLA-bioresponsive markers. When cells were incubated in the presence of VA at concentrations of 5 to 20 μg/mL, both VA and c9,t11-CLA increased in cellular lipids in a dose-dependent manner. After 4 d of incubation of SW480 and MCF-7 cells with VA (20 μg/mL), c9,t11-CLA increased from undetectable levels to 8.57 and 12.14 g/100 g FAME in cellular lipids, respectively. VA supplementation for 4 d at 5, 10, and 15 μg/mL had no effect on cell growth, whereas 20 μg/mL significantly (P<0.05) reduced cell growth in both cell lines. VA (20 μg/mL) treatment induced DNA fragmentation and significantly (P<0.05) depeleted cytosolic GSH levels in the SW480 cell line after 4 d of incubation, suggesting that apoptosis was the mode of cell death induced by VA. Both VA and c9,t11-CLA reduced (P<0.05) total ras expression in SW480 cells. 14C-Arachidonic acid uptake into the MG fraction was significantly increased (P<0.05) in both cell lines while uptake into the phospholipid fraction decreased in response to VA. VA treatment significantly (P<0.05) increased 8-epi-prostaglandin F2α in both cell lines. The data indicate that growth suppression and cellular responses of both cells lines are likely mediated by VA desaturation to c9,t11-CLA via Δ9-desaturase.

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Abbreviations

AA:

arachidonic acid (20∶4)

HRP:

horseradish peroxidase

LA:

linoleic acid (18∶2)

MG:

monoacylglyceride

PBST:

PBS containing 0.1% (by vol) Tween 20

PGD2 :

prostaglandin D2

PGE2 :

prostaglandin E2

PGF2α :

prostaglandin F2α

PL:

phospholipid

TG:

triacylglyceride

VA:

vaccenic acid (t11–18∶1)

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Authors and Affiliations

  1. School of Biotechnology, Dublin City University, 9, Dublin, Ireland

    Aine Miller & Emma McGrath

  2. Dairy Products Research Centre, Teagasc, Moorepark, Fermoy, Co. Cork, Ireland

    Emma McGrath & Catherine Stanton

  3. National Institute of Cellular Biotechnology, School of Biotechnology, Dublin City University, 9, Dublin, Ireland

    Rosaleen Devery

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  1. Aine Miller
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  2. Emma McGrath
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  3. Catherine Stanton
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  4. Rosaleen Devery
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Correspondence to Rosaleen Devery.

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Miller, A., McGrath, E., Stanton, C. et al. Vaccenic acid (t11–18∶1) is converted to c9,t11-CLA in MCF-7 and SW480 cancer cells. Lipids 38, 623–632 (2003). https://doi.org/10.1007/s11745-003-1107-8

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  • DOI: https://doi.org/10.1007/s11745-003-1107-8

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